Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection

• Published in: Diagnostics (Basel) Vol. 13; no. 5; p. 846
• Main Authors: Mijač, Maja, Ljubin-Sternak, Sunčanica, Ivković-Jureković, Irena, Vraneš, Jasmina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23-02-2023; MDPI
• Subjects: Adenoviruses; Causes of; children; Comparative analysis; Coronaviruses; Diagnosis; human bocavirus; Identification and classification; Influenza; MT-PCR; Parvoviruses; Pathogens; Patients; Polymerase chain reaction; Process controls; Respiratory syncytial virus; respiratory tract; Respiratory tract infections; Testing; Viral infections; viral load; Viruses; Croatia; MT-PCR; viral load; children; respiratory tract; human bocavirus
• ISSN: 2075-4418; 2075-4418
• DOI: 10.3390/diagnostics13050846

Human bocavirus (HBoV) is an important respiratory pathogen, especially in children, but it is often found in co-detection with other respiratory viruses, which makes the diagnostic approach challenging. We compared multiplex PCR and quantitative PCR for HBoV with multiplex tandem PCR (MT-PCR) in 55 cases of co-detection of HBoV and other respiratory viruses. In addition, we investigated whether there is a connection between the severity of the disease, measured by the localization of the infection, and amount of virus detected in the respiratory secretions. No statistically significant difference was found, but children with large amount of HBoV and other respiratory virus had a longer stay in hospital.