Application of curve resolution algorithms in the study of drug photodegradation kinetics -- the example of moclobemide

• Published in: Journal of AOAC International Vol. 95; no. 3; pp. 708 - 712
• Main Authors: Skibinski, Robert, Komsta, Lukasz
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-05-2012
• Subjects: Algorithms; Benzamides - chemistry; Chemical properties; Chromatography, High Pressure Liquid; Computational chemistry; Identification and classification; Kinetics; Moclobemide; Moclobemide - chemistry; Optical properties; Pharmacokinetics; Photodegradation; Photolysis; Tandem Mass Spectrometry; Poland
• ISSN: 1060-3271; 1944-7922
• DOI: 10.5740/jaoacint.SGE_Skibinski

The photodegradation of moclobemide was studied in methanolic media. Ultra-HPLC (UHPLC)/MS/MS analysis proved decomposition to 4-chlorobenzamide as a major degradation product and small amounts of Ro 16-3177 (4-chloro-N-[2-[(2-hydroxyethyl)amino] ethyl]benzamide) and 2-[(4-chlorobenzylidene)amino]-N-[2-ethoxyethenyl]ethenamine. The methanolic solution was investigated spectrophotometrically in the UV region, registering the spectra during 30 min of degradation. Using reference spectra and a multivariate chemometric method (multivariate curve resolution-alternating least squares), the spectra were resolved and concentration profiles were obtained. The obtained results were in good agreement with a quantitative approach, with UHPLC-diode array detection as the reference method.