Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment

• Published in: Diagnostics (Basel) Vol. 13; no. 1; p. 140
• Main Authors: Ivanov, Mikhail K, Brenner, Evgeny V, Hodkevich, Anastasia A, Dzyubenko, Victoria V, Krasilnikov, Sergey E, Mansurova, Alphiya S, Vakhturova, Irina E, Agletdinov, Eduard F, Shumeikina, Anastasia O, Chernyshova, Alyona L, Titov, Sergei E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-01-2023; MDPI
• Subjects: 16S rRNA gene sequencing; Age; Cancer; Cancer therapies; cancer treatment; Care and treatment; Cellular biology; Cervical cancer; cervicovaginal microbiome; Clinics; Health aspects; high-grade squamous intraepithelial lesion; Human papillomavirus; Medical screening; Microbiota (Symbiotic organisms); Patient outcomes; Polymerase chain reaction; post-treatment effect; Regions; Thermal cycling; Vagina; Womens health; Russia; United States > US; Lactobacillus iners; cancer treatment; cervicovaginal microbiome; Cutibacterium acnes; post-treatment effect; human papillomavirus; 16S rRNA gene sequencing; high-grade squamous intraepithelial lesion; real-time PCR; cervical cancer
• DOI: 10.3390/diagnostics13010140
• EISSN: 2075-4418

Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)-especially
spp.,
spp., and
-was low in post-treatment patients and depended on treatment types. Frequency of
dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes.