Pharmacokinetics, food intake requirements and tolerability of once‐daily combinations of nelfinavir and low‐dose ritonavir in healthy volunteers

• Published in: British journal of clinical pharmacology Vol. 55; no. 2; pp. 115 - 125
• Main Authors: Aarnoutse, R. E., Droste, J. A. H., Van Oosterhout, J. J. G., Koopmans, P. P., Popescu, M., Reiss, P., Hekster, Y. A., Burger, D. M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-02-2003; Blackwell Science; Blackwell Science Inc
• Subjects: Adolescent; Adult; Aged; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Biological and medical sciences; Drug Administration Schedule; Eating - physiology; Female; Half-Life; HIV Protease Inhibitors - administration & dosage; HIV Protease Inhibitors - adverse effects; HIV Protease Inhibitors - pharmacokinetics; Humans; Male; Medical sciences; Middle Aged; nelfinavir; Nelfinavir - administration & dosage; Nelfinavir - adverse effects; Nelfinavir - pharmacokinetics; once daily; Pharmacokinetics; Pharmacology. Drug treatments; ritonavir; Ritonavir - administration & dosage; Ritonavir - adverse effects; Ritonavir - pharmacokinetics; Human; Drug combination; Healthy subject; Ritonavir; Single dose; Nelfinavir; Oral administration; Antiviral; once daily; Daily dose; Pharmacokinetics; Food drug interaction
• ISSN: 0306-5251; 1365-2125
• DOI: 10.1046/j.1365-2125.2003.01756.x

Aims This study was performed to evaluate the steady‐state pharmacokinetics, food intake requirements and short‐term tolerability of once‐daily combinations of nelfinavir and low‐dose ritonavir.
Methods Twenty‐seven healthy volunteers were randomized over three groups to receive a once‐daily regimen of nelfinavir/ritonavir 2000/200 mg (group 1), 2000/400 mg (group 2) or 2500/200 mg (group 3) with food for 14 days. Pharmacokinetic parameters for nelfinavir and its active metabolite M8 were assessed on study days 15 and 16, after administration of the regimens with a full (610 kcal) or light (271 kcal) breakfast, respectively.
Results Pharmacokinetic data were evaluable for eight volunteers in group 1, eight in group 2 and four in group 3. Administration of nelfinavir/ritonavir with a full breakfast resulted in geometric mean (GM) nelfinavir AUC24h values of 76.8, 51.3, and 61.9 h*mg/l in group 1, 2 and 3, respectively. GM 24‐h Cmin concentrations of nelfinavir were 0.76 mg l−1, 0.43 mg l−1 and 0.47 mg l−1, respectively. Co‐administration of ritonavir increased M8 concentrations more than nelfinavir concentrations, resulting in GM AUC24h and Cmin values for nelfinavir plus M8 that were higher than or comparable to reference values for the approved regimen of nelfinavir (1250 mg BID without ritonavir). In the 2000/200 mg group, seven out of eight subjects had a Cmin value of nelfinavir plus M8 above a threshold of 1.0 mg l−1. Administration of the combinations with a light breakfast resulted in significant decreases in the AUC24h and Cmin of nelfinavir and nelfinavir plus M8, compared with intake with a full breakfast. For the Cmin of nelfinavir plus M8, the GM ratio (light/full breakfast) was 0.76 (90% confidence interval 0.67–0.86, participants from all groups combined). Short‐term tolerability was satisfactory, apart from a higher than expected incidence of mild rash (12%).
Conclusions Administration of nelfinavir in a once‐daily regimen appears feasible. A nelfinavir/ritonavir 2000/200 mg combination appears appropriate for further evaluation. Once‐daily nelfinavir/ritonavir should be taken with a meal containing at least 600 kcal.