Validation of an ion trap tandem mass spectrometric analysis of mulberry 1-deoxynojirimycin in human plasma: Application to pharmacokinetic studies

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 8; pp. 2210 - 2213
• Main Authors: Nakagawa, K.(Tohoku Univ., Sendai (Japan)), Kubota, H, Tsuzuki, T, Kariya, J, Kimura, T, Oikawa, S, Miyazawa, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-08-2008; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: 1-deoxynojirimycin; 1-Deoxynojirimycin - blood; 1-Deoxynojirimycin - chemistry; 1-Deoxynojirimycin - pharmacokinetics; ABSORCION; ABSORPTION; Biological and medical sciences; BLOOD PLASMA; DIABETE; DIABETES; ESPECTROMETRIA DE MASAS; Fundamental and applied biological sciences. Psychology; human plasma; Humans; INSULIN; INSULINA; INSULINE; Ions - chemistry; MASS SPECTROMETRY; Molecular Structure; MORA; Morus - chemistry; MULBERRIES; mulberry; MURE; ORINA; PLASMA SANGUIN; PLASMA SANGUINEO; SPECTROMETRIE DE MASSE; Tandem Mass Spectrometry; URINE; Human; Validation; Absorption; tandem mass spectrometry; Mass spectrometry MS/MS; 1-deoxynojirimycin; mulberry; Pharmacokinetics; Application; Mass spectrometry; human plasma
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80200

Mulberry 1-deoxynojirimycin (DNJ) is a potent alpha-glucosidase inhibitor. Although it is useful for the treatment of diabetes, the human absorption and metabolism of DNJ have never been characterized. We developed a method using hydrophilic interaction chromatography coupled with ion trap tandem mass spectrometry, and found that orally administered DNJ was absorbed into the blood and then excreted into the urine.