Proposal of standardized methods and reference for assaying recombinant human tumor necrosis factor

Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the t...

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Bibliographic Details
Published in:	Japanese journal of medical science & biology Vol. 39; no. 3; p. 105
Main Authors:	Yamazaki, S, Onishi, E, Enami, K, Natori, K, Kohase, M, Sakamoto, H, Tanouchi, M, Hayashi, H
Format:	Journal Article
Language:	English
Published:	Japan 1986
Subjects:	Antibodies, Monoclonal Biological Assay Cell Survival - drug effects Enzyme-Linked Immunosorbent Assay Freeze Drying Glycoproteins - immunology Glycoproteins - pharmacology Glycoproteins - standards Humans L Cells (Cell Line) Tumor Necrosis Factor-alpha
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Summary:	Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the two assay methods were found appropriate for standardization of rH-TNF. A freeze-dried reference was prepared, and examination of its potency and stability showed it to be suitable for use as a reference standard for rH-TNF assays.
ISSN:	0021-5112
DOI:	10.7883/yoken1952.39.105