Changes in composition and content of mycolic acids in glutamate-overproducing Corynebacterium glutamicum

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 1; pp. 22 - 30
• Main Authors: Hashimoto, K.(Tokyo Denki Univ., Tokyo (Japan)), Kawasaki, H, Akazawa, K, Nakamura, J, Asakura, Y, Kudo, T, Sakuradani, E, Shimizu, S, Nakamatsu, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-01-2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACIDE GLUTAMIQUE; ACIDE GRAS; ACIDO GLUTAMICO; ACIDOS GRASOS; ALIMENT FERMENTE; ALIMENTOS FERMENTADOS; Biological and medical sciences; BIOTIN; BIOTINA; BIOTINE; CELL CULTURE; Cerulenin - pharmacology; Chromatography, Gas; CORYNEBACTERIUM; Corynebacterium glutamicum; Corynebacterium glutamicum - chemistry; Corynebacterium glutamicum - drug effects; Corynebacterium glutamicum - metabolism; CULTIVO DE CELULAS; CULTURE DE CELLULE; Esters - chemistry; fatty acid; FATTY ACIDS; FERMENTED FOODS; Fundamental and applied biological sciences. Psychology; glutamate overproduction; Glutamates - biosynthesis; GLUTAMIC ACID; Mass Spectrometry; Methylation; Molecular Structure; mycolic acid; Mycolic Acids - chemistry; Mycolic Acids - metabolism; glutamate overproduction; mycolic acid; Corynebacterium glutamicum; fatty acid; Bacteria; Actinomycetes; Corynebacteriaceae; Glutamate; Fatty acids
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.70.22

Overproduction of glutamate by Corynebacterium glutamicum is induced by biotin limitation or by the supplementation of specific detergents, sublethal amounts of penicillin, or cerulenin. But, it remains unclear why these different treatments, which have different sites of primary action, produce similar effects. In this study, it was found that the cellular content of mycolic acids-characteristic constituents of Corynebacterineae that are synthesized from fatty acids and form a cell surface layer-decreased under all conditions that induced glutamate overproduction. Furthermore, short mycolic acids increased under conditions of biotin limitation and cerulenin supplementation. These results suggest that different treatments produce the same effect that causes defects in the mycolic acid layer. This is perhaps one of the key factors in overproduction of glutamate by C. glutamicum.