Butanol production by Clostridium beijerinckii ATCC 55025 from wheat bran

• Published in: Journal of industrial microbiology & biotechnology Vol. 37; no. 5; pp. 495 - 501
• Main Authors: Liu, Ziyong, Ying, Yu, Li, Fuli, Ma, Cuiqing, Xu, Ping
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-05-2010; Springer-Verlag; Springer; Oxford University Press
• Subjects: Acetone; Acetone - metabolism; Biochemistry; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Butanols - metabolism; Carbon; Clostridium beijerinckii; Clostridium beijerinckii - metabolism; Dietary fiber; Dietary Fiber - metabolism; Ethanol; Ethanol - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Genetic Engineering; Glucose; Glucose - metabolism; Industrial Microbiology; Inorganic Chemistry; Laboratories; Life Sciences; Microbiology; Moisture content; Original Paper; Renewable resources; Starch - metabolism; Studies; Sulfuric acid; Sulfuric Acids - metabolism; Triticum aestivum; Wheat; Wheat bran; Xylose - metabolism; Butanol; Dilute sulfuric acid pretreatment; Wheat bran; ATCC 55025; Cereal bran; Clostridium beijerinckii ATCC 55025; Clostridiaceae; Production; Clostridiales; Bacteria; Pretreatment; Clostridium beijerinckii; Sulfuric acid
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-010-0695-8

Wheat bran, a by-product of the wheat milling industry, consists mainly of hemicellulose, starch and protein. In this study, the hydrolysate of wheat bran pretreated with dilute sulfuric acid was used as a substrate to produce ABE (acetone, butanol and ethanol) using Clostridium beijerinckii ATCC 55025. The wheat bran hydrolysate contained 53.1 g/l total reducing sugars, including 21.3 g/l of glucose, 17.4 g/l of xylose and 10.6 g/l of arabinose. C. beijerinckii ATCC 55025 can utilize hexose and pentose simultaneously in the hydrolysate to produce ABE. After 72 h of fermentation, the total ABE in the system was 11.8 g/l, of which acetone, butanol and ethanol were 2.2, 8.8 and 0.8 g/l, respectively. The fermentation resulted in an ABE yield of 0.32 and productivity of 0.16 g l⁻¹ h⁻¹. This study suggests that wheat bran can be a potential renewable resource for ABE fermentation.