invertebrate-like phototransduction cascade mediates light detection in the chicken retinal ganglion cells

• Published in: The FASEB journal Vol. 20; no. 14; pp. 2648 - 2650
• Main Authors: Contin, Maria Ana, Verra, Daniela M, Guido, Mario E
• Format: Journal Article
• Language: English
• Published: United States The Federation of American Societies for Experimental Biology 01-12-2006; Federation of American Societies for Experimental Biology
• Subjects: Animals; Cells, Cultured; Chick Embryo; Chordata; Gene Expression Regulation; Invertebrates; Light; Light Signal Transduction - drug effects; Light Signal Transduction - physiology; Light Signal Transduction - radiation effects; melatonin; Melatonin - metabolism; phosphoinositide cascade; Retinal Ganglion Cells - metabolism; RGCs; rhabdomeric photoreceptors; Tritium; Vitamin A - chemistry; Vitamin A - metabolism
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.06-6133fje

Prebilaterian animals perceived ambient light through nonvisual rhabdomeric photoreceptors (RPs), which evolved as support of the chordate visual system. In vertebrates, the identity of nonvisual photoreceptors and the phototransduction cascade involved in nonimage forming tasks remain uncertain. We investigated whether chicken retinal ganglion cells (RGCs) could be nonvisual photoreceptors and the nature of the photocascade involved. We found that primary cultures of chicken embryonic RGCs express such RP markers as transcription factors Pax6 and Brn3, photopigment melanopsin, and G-protein q but not markers for ciliary photoreceptors (α-transducin and Crx). To investigate the photoreceptive capability of RGCs, we assessed the direct effect of light on ³H-melatonin synthesis in RGC cultures synchronized to 12:12 h light-dark cycles. In constant dark, RGCs displayed a daily variation in ³H-melatonin levels peaking at subjective day, which was significantly inhibited by light. This light effect was further increased by the chromophore all-trans-retinal and suppressed by specific inhibitors of the invertebrate photocascade involving phosphoinositide hydrolysis (100 μM neomycin; 5 μM U73122) and Ca²⁺ mobilization (10 mM BAPTA; 1 mM lanthanum). The results demonstrate that chicken RGCs are intrinsically photosensitive RPs operating via an invertebrate-like phototransduction cascade, which may be responsible for early detection of light before vision occurs.--Contin, M. A., Verra, D. M., Guido, M. E. An invertebrate-like phototransduction cascade mediates light detection in the chicken retinal ganglion cells