Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation

• Published in: Bone marrow transplantation (Basingstoke) Vol. 45; no. 11; pp. 1638 - 1644
• Main Authors: Jacewicz, R, Lewandowski, K, Rupa-Matysek, J, Jedrzejczyk, M, Brzezinski, P M, Dobosz, T, Jonkisz, A, Szram, S, Komarnicki, M, Berent, J
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01-11-2010; Nature Publishing Group
• Subjects: 631/136/2128; 631/45/147; 692/700/565/545/576/1955; Adult; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Bone marrow; Bone marrow, stem cells transplantation. Graft versus host reaction; Care and treatment; Cell Biology; Chemotherapy; Chimerism; Chromosomes, Human, Y; Deoxyribonucleic acid; DNA; DNA - analysis; DNA - genetics; Female; Fluorescence; Follicles; Graft Survival; Graft versus host reaction; Hair; Hair Follicle - chemistry; Hair Follicle - physiology; Hematology; Hematopoietic Stem Cell Transplantation; Hematopoietic stem cells; Humans; Internal Medicine; Male; Medical examination; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Organ transplant recipients; original-article; Polymerase chain reaction; Polymerase Chain Reaction - methods; Prognosis; Public Health; Risk factors; Stem cell transplantation; Stem Cells; Tissue Donors; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation; Transplantation Chimera; Y chromosomes; Young Adult; Poland; hair follicles chimerism; SRY; sex-mismatched allografts; HSCT; Y-STR; Human; Incompatibility; Hematology; Stem cell; Sex; Hematopoietic cell; Homograft; Hair follicle; Recipient; Tandemly repeated sequence; Population genetics; Allogeneic hematopoietic stem cell transplantation; Donor; Microsatellite DNA; Chimerism; Hematopoietic stem cell transplantation; Testis-determining factor
• ISSN: 0268-3369; 1476-5365
• DOI: 10.1038/bmt.2010.27

The hair follicles of recipients of allogeneic hematopoietic SCT (HSCT) constitute the tissue with the greatest need for regeneration after high-dose chemotherapy. Previous studies have shown a lack of donor-derived DNA in the hair follicles of recipients. Therefore, we carried out a study to determine whether male donor-derived genetic material can be found in female recpients’ hair follicles after HSCT. Fluorescent-based PCR with analyses of Y-chromosome STR (Y-STR) and RQ-PCR with the sex-determining region Y (SRY) were used independently to evaluate chimerism status. Our results proved the existence of donor-derived stem DNA in the recipients’ hair follicle cells. This report undermines the validity of data indicating that hair follicle cells maintain 100% of recipient origin.