Application of PCR to Distinguish Common Species of Dermatophytes

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Published in:	Journal of Clinical Microbiology Vol. 39; no. 9; pp. 3382 - 3385
Main Authors:	FAGGI, Elisabetta, PINI, Gabriella, CAMPISI, Enza, BERTELLINI, Chiara, DIFONZO, Elisa, MANCIANTI, Francesca
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-09-2001
Series:	Note
Subjects:	Animals Arthrodermataceae - classification Arthrodermataceae - genetics Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cats Dermatomycoses - microbiology DNA Primers DNA, Fungal - analysis DNA, Fungal - isolation & purification Dogs Epidermophyton - classification Epidermophyton - genetics Epidermophyton floccosum Fundamental and applied biological sciences. Psychology Humans Microbiology Microsporum - classification Microsporum - genetics Microsporum canis Microsporum gypseum Mycology oligonucleotides Polymerase Chain Reaction - methods Techniques used in virology Trichophyton - classification Trichophyton - genetics Trichophyton ajelloi Trichophyton mentagrophytes Trichophyton rubrum Virology Polymerase chain reaction Dermatophyte
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Abstract	Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM
AbstractList	This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA) 4 . The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis , Microsporum gypseum , Trichophyton rubrum , Trichophyton ajelloi , and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group. This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA)(4). The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton ajelloi, and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group. This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA) sub(4). The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton ajelloi, and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group. Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM
Author	Enza Campisi Francesca Mancianti Elisabetta Faggi Elisa Difonzo Chiara Bertellini Gabriella Pini
AuthorAffiliation	Dipartimento di Sanità Pubblica—Sezione Microbiologia, Università di Firenze, 50134 Florence, 1 Dipartimento di Scienze Dermatologiche, Università di Firenze, 50121 Florence, 2 and Dipartimento di Patologia Animale, Università di Pisa, 56124 Pisa, 3 Italy
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SubjectTerms	Animals Arthrodermataceae - classification Arthrodermataceae - genetics Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cats Dermatomycoses - microbiology DNA Primers DNA, Fungal - analysis DNA, Fungal - isolation & purification Dogs Epidermophyton - classification Epidermophyton - genetics Epidermophyton floccosum Fundamental and applied biological sciences. Psychology Humans Microbiology Microsporum - classification Microsporum - genetics Microsporum canis Microsporum gypseum Mycology oligonucleotides Polymerase Chain Reaction - methods Techniques used in virology Trichophyton - classification Trichophyton - genetics Trichophyton ajelloi Trichophyton mentagrophytes Trichophyton rubrum Virology
Title	Application of PCR to Distinguish Common Species of Dermatophytes
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