Transplantation of photobiomodulation-preconditioned diabetic stem cells accelerates ischemic wound healing in diabetic rats

• Published in: Stem cell research & therapy Vol. 11; no. 1; p. 494
• Main Authors: Ahmadi, Houssein, Amini, Abdollah, Fadaei Fathabady, Fatemeh, Mostafavinia, Atarodsadat, Zare, Fatemeh, Ebrahimpour-Malekshah, Roohollah, Ghalibaf, Mustafa Neshat, Abrisham, Matin, Rezaei, Fatemehalsadat, Albright, Richard, Ghoreishi, Seyed Kamran, Chien, Sufan, Bayat, Mohammad
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25-11-2020; BioMed Central
• Subjects: Advertising executives; Animals; Apoptosis; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - therapy; Diabetic foot; Foot diseases; Glucose; Humans; Hypoxia; Inflammation; Ischemia; Lasers; Light therapy; Low-Level Light Therapy; Medical research; Medicine, Experimental; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchyme; Methicillin; Rats; Rats, Wistar; Rodents; Skin; Staphylococcus aureus infections; Staphylococcus infections; Stem cell transplantation; Stem Cells; Surgery; Transplantation; Type 1 diabetes; Wound Healing; Adipose-derived mesenchymal stem cells; Methicillin-resistant Staphylococcus aureus infection; Wound healing; Wound closure rate; Preconditioning stem cell, photobiomodulation; Rats; Tensiometric properties; Diabetes mellitus Ischemia
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-020-01967-2

Diabetic foot ulcer is the most costly and complex challenge for patients with diabetes. We hereby assessed the effectiveness of different preconditioned adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation protocols on treating an infected ischemic wound in type 1 diabetic rats.
There were five groups of rats: (1) control, (2) control AD-MSCs [diabetic AD-MSCs were transplanted (grafted) into the wound bed], (3) AD-MSC + photobiomodulation in vivo (diabetic AD-MSCs were grafted into the wound, followed by in vivo PBM treatment), (4) AD-MSCs + photobiomodulation in vitro, and (5) AD-MSCs + photobiomodulation in vitro + in vivo.
Diabetic AD-MSCs preconditioned with photobiomodulation had significantly risen cell function compared to diabetic AD-MSC. Groups 3 and 5 had significantly decreased microbial flora correlated to groups 1 and 2 (all, p = 0.000). Groups 2, 3, 4, and 5 had significantly improved wound closure rate (0.4, 0.4, 0.4, and 0.8, respectively) compared to group 1 (0.2). Groups 2-5 had significantly increased wound strength compared to group 1 (all p = 0.000). In most cases, group 5 had significantly better results than groups 2, 3, and 4.
Preconditioning diabetic AD-MSCs with photobiomodulation in vitro plus photobiomodulation in vivo significantly hastened healing in the diabetic rat model of an ischemic infected delayed healing wound.