Modulation of Survival and Proliferation of BSC-1 Cells through Changes in Spreading Behavior Caused by the Tumor-Promoting Phorbol Ester TPA

• Published in: Cell Structure and Function Vol. 14; no. 6; pp. 685 - 696
• Main Authors: Shiba, Yoshiki, Kanno, Yoshinobu
• Format: Journal Article
• Language: English
• Published: Kyoto Japan Society for Cell Biology 1989; Japan Science and Technology Agency
• Subjects: Animal cells; Animals; Biological and medical sciences; Cell Adhesion - drug effects; Cell cultures. Hybridization. Fusion; Cell Division - drug effects; Cell Line; Cell Survival - drug effects; Culture Media; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0386-7196; 1347-3700
• DOI: 10.1247/csf.14.685

The effect of a tumor-promoting phorbol ester on spreading behavior was investigated to clarify the involvement of the interactions between cells and substratum in the maintenance of cell viability and the control of cell proliferation. BSC-1 cells did not spread and lost cell viability after a 24-h in-cubation in the absence of calf serum. Addition of calf serum initially induced radial spreading and then polarized spreading, with the formation on stress fibers and focal contact-like structure, and enhanced survival. Vitronectin also induced both radial spreading and polarized spreading, and enhanced cell sur-vival. 12-O-Tetradecanoylphorbol-13-acetate (TPA) induced radial spreading with actin ribbons in the absence of serum. It improved the survival of cells at-tached to the substratum, but not in suspension. TPA suppressed polarized spreading, formation of stress fibers and of focal contact-like structure, and cell proliferation, in the presence of serum. Phorbol did not have any effect. These results suggest that enhancement of radial spreading and inhibition of polarized spreading of BSC-1 cells by TPA are closely related to the enhancement of cell survival and inhibition of cell growth.