超支化聚胺-酯改性聚二甲基硅氧烷微流控芯片的紫外检测应用

超支化聚胺-酯改性聚二甲基硅氧烷微流控芯片的紫外检测应用

采用超支化聚胺-酯对经过氧气氛处理的PDMS微流控芯片表面进行改性。成功地将超支化聚胺-酯涂覆到PDMS表面,使其表面的接触角由108°±1°降到32°±2°,改善了其亲水性;改性过后通道内的电渗流得到了有效抑制,远低于未改性通道内的电渗流。同时,将芯片通过专门设计的通道与毛细管连接在一起,在紫外检测波长214 nm,分离电压5 kV,pH 4.5,电动进样条件下,成功实现了对腺苷和L-色氨酸的紫外检测分离。改性后的芯片对检测物质的分离度可以达到2.01,信号重现性RSD（n=4）分别为2.75%（腺苷）和1.54%（L-色氨酸）。本方法为改性PDMS微流控芯片提供了一种新方法。...

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Bibliographic Details
Published in:分析化学 Vol. 39; no. 6; pp. 890 - 893
Main Author: 杨文 林栋 徐磊 刘冰 寿崇琦
Format: Journal Article
Language:Chinese
Published: 济南大学化学化工学院,济南,250022 2011
Subjects:
UV检测
微流控芯片
表面改性
超支化聚胺-酯
微流控芯片
UV检测
超支化聚胺-酯
表面改性
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Summary:采用超支化聚胺-酯对经过氧气氛处理的PDMS微流控芯片表面进行改性。成功地将超支化聚胺-酯涂覆到PDMS表面,使其表面的接触角由108°±1°降到32°±2°,改善了其亲水性;改性过后通道内的电渗流得到了有效抑制,远低于未改性通道内的电渗流。同时,将芯片通过专门设计的通道与毛细管连接在一起,在紫外检测波长214 nm,分离电压5 kV,pH 4.5,电动进样条件下,成功实现了对腺苷和L-色氨酸的紫外检测分离。改性后的芯片对检测物质的分离度可以达到2.01,信号重现性RSD（n=4）分别为2.75%（腺苷）和1.54%（L-色氨酸）。本方法为改性PDMS微流控芯片提供了一种新方法。
Bibliography:Hyperbranched polyaminoesters（HPAE） were used for poly（dimethylsiloxane）（PDMS） microfluidic chips modification.The microdevices were treated in oxygen atmosphere before modification.The contact angles of PDMS were lowered from 108°±1° to 32°±2° in this method.The hydrophilcity of PDMS was improved.The electroosmotic flow（EOF） in the modified microchannel was lower than that in the unmodified microchannel.The EOF in the modified microchannel was effectively inhibited.A fused-silica capillary was connected to the chip through the specially designed channel.With this method,detection wavelength 214 nm,running voltage 5 kV,pH 4.5 and electrokinetic injection,UV detection for adenosine and L-tryptophan was achieved.The Rs of adenosine and L-tryptophan in modified microchannel can achieve 2.01.The RSD is 2.75%（Adenosine） and 1.54%（L-tryptophan） respectively.This research may be a novel method for PDMS microchip modification.
Microfluidic chip; Hyperbranched polyaminoester; Surface modification; Ultraviolet detection
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2011.00890