Development and Validation of a UPLC-MS/MS Method for Therapeutic Drug Monitoring, Pharmacokinetic and Stability Studies of First-Line Antituberculosis Drugs in Urine

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 2; p. 337
• Main Authors: Abouzid, Mohamed, Kosicka-Noworzyń, Katarzyna, Karaźniewicz-Łada, Marta, Rao, Prakruti, Modi, Nisha, Xie, Yingda L, Heysell, Scott K, Główka, Anna, Kagan, Leonid
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-01-2024; MDPI
• Subjects: Accuracy; Acetonitrile; Acquired immune deficiency syndrome; AIDS; Antitubercular Agents - therapeutic use; Backup software; Calibration; Chromatography; Chromatography, Liquid; Coronaviruses; Drug Monitoring; Drugs; Ethambutol; Formic acid; Health aspects; Humans; isoniazid; Liquid Chromatography-Mass Spectrometry; Mass spectrometry; Massachusetts; Medical research; Medicine, Experimental; Metabolites; Methods; Mortality; New Jersey; Organic acids; Pharmacokinetics; Plasma; pyrazinamide; rifampicin; Rifampin; Rifampin - therapeutic use; Tandem Mass Spectrometry; Therapeutic drug monitoring; Tuberculosis; Urine; Massachusetts; New Jersey; rifampicin; isoniazid; therapeutic drug monitoring; ethambutol; pyrazinamide
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29020337

Tuberculosis (TB) remains one of the leading global causes of mortality. Several methods have been established to detect anti-TB agents in human plasma and serum. However, there is a notable absence of studies analyzing TB drugs in urine. Thus, our objective was to validate a method for quantifying first-line anti-TB agents: isoniazid (INH), pyrazinamide (PZA), ethambutol (ETH), and rifampicin (RIF), along with its metabolite 25-desacetylrifampicin, and degradation products: rifampicin quinone and 3-formyl-rifampicin in 10 µL of urine. Chromatographic separation was achieved using a Kinetex Polar C18 analytical column with gradient elution (5 mM ammonium acetate and acetonitrile with 0.1% formic acid). Mass spectrometry detection was carried out using a triple-quadrupole tandem mass spectrometer operating in positive ion mode. The lower limit of quantification (LLOQ) was 0.5 µg/mL for INH, PZA, ETH, and RIF, and 0.1 µg/mL for RIF's metabolites and degradation products. The method was validated following FDA guidance criteria and successfully applied to the analysis of the studied compounds in urine of TB patients. Additionally, we conducted a stability study of the anti-TB agents under various pH and temperature conditions to mimic the urine collection process in different settings (peripheral clinics or central laboratories).