The role of micro RNAs let7c, 100 and 218 expression and their target RAS, C-MYC, BUB1, RB, SMARCA5, LAMB3 and Ki-67 in prostate cancer

• Published in: Clinics (São Paulo, Brazil) Vol. 68; no. 5; pp. 652 - 657
• Main Authors: Reis, Sabrina T., Timoszczuk, Luciana S., Pontes-Junior, José, Viana, Nayara, Silva, Iran A., Dip, Nelson, Srougi, Miguel, Leite, Katia R.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier España, S.L.U 01-05-2013; Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo; Faculdade de Medicina / USP; Elsevier España
• Subjects: Adenosine Triphosphatases - metabolism; Adult; Bone Neoplasms - secondary; BUB1; C-MYC; Cell Adhesion Molecules - metabolism; Chromosomal Proteins, Non-Histone - metabolism; Clinical Science; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Kalinin; Ki-67 Antigen - metabolism; Ki-67, RB; LAMB3; Lymphatic Metastasis; Male; MEDICINE, GENERAL & INTERNAL; Micro RNA; MicroRNAs - genetics; MicroRNAs - metabolism; MicroRNAs - physiology; Middle Aged; Neoplasm Proteins - metabolism; Neoplasm Proteins - physiology; Prognosis; Prostate Cancer; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Protein Serine-Threonine Kinases - metabolism; Proto-Oncogene Proteins c-myc - metabolism; Proto-Oncogene Proteins p21(ras) - metabolism; RAS; Retinoblastoma Protein - metabolism; SMARCA5; Tumor Markers; Immunohistochemistry; Prognosis; LAMB3; RAS; Ki-67, RB; Micro RNA; C-MYC; SMARCA5; Prostate Cancer; Tumor Markers; BUB1
• ISSN: 1807-5932; 1980-5322; 1980-5322
• DOI: 10.6061/clinics/2013(05)12

The aim of this study is to verify the expression of proteins that are controlled by miR-let7c, 100 and 218 using immunohistochemistry in tissue microarray representative of localized and metastasized the lymph nodes and bone prostate cancer.
To verify the expression of proteins that are controlled by miR-let7c (C-MYC, BUB1, RAS) 100 (SMARCA5, RB) and 218 (LAMB3) and cell proliferation (Ki-67) we used immunohistochemistry and computerized image system ImageJ MacBiophotonics in three tissue microarrays representative of localized prostate cancer and lymph node and bone metastases. miRNA expression was evaluated by qRT-PCR using 60 paraffin blocks to construct the tissue microarray representative of localized disease.
RAS expression was increased in localized prostate cancer and bone metastases compared to the lymph nodes (p = 0.017). RB showed an increase in expression from localized prostate cancer to lymph node and bone metastasis (p = 0.036). LAMB3 was highly expressed in localized and lymph node metastases (p<0.001). Cell proliferation evaluated by Ki-67 showed an increase from localized prostate cancer to metastases (p<0.001). We did not found any relationship between C-MYC (p = 0.253), BUB1 (p = 0.649) and SMARCA5 (p = 0.315) protein expression with prognosis or tumor behavior.
We found that the expression of RAS, RB, LAMB3 and Ki-67 changed in the different stages of prostate cancer. Furthermore, we confirmed the overexpression of the miRNAs let7c, 100 and 218 in localized prostate cancer but failed to show the control of protein expression by the putative controller miRNAs using immunohistochemistry.