LpA-II:B:C:D:E: a new immunochemically-defined acute phase lipoprotein in humans

• Published in: Lipids in health and disease Vol. 17; no. 1; p. 127
• Main Authors: Bagdade, John D, Jilma, Bernd, Hudgins, Lisa C, Alaupovic, Petar, McCurdy, Carrie E
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28-05-2018; BioMed Central; BMC
• Subjects: Acute phase proteins; Apolipoproteins; Arthritis; Atherosclerosis; Cholesterol; Cytokines; Endotoxemia; Endotoxin, Inflammation, Lipoprotein subclasses, TGLRP, triglyceride—rich lipoproteins, Innate immunity; Endotoxins; Genetic aspects; Immune response; Immunoregulation; Infections; Inflammation; Lipids; Lipoproteins; Low density lipoprotein; Metabolism; Physiological aspects; Post-translation; Proteins; Rodents; Sepsis; Studies; Ultracentrifugation; United States; New York; United States > US; Endotoxin, Inflammation, Lipoprotein subclasses, TGLRP, triglyceride—rich lipoproteins, Innate immunity
• ISSN: 1476-511X; 1476-511X
• DOI: 10.1186/s12944-018-0769-6

Previous studies of lipoproteins in patients with sepsis have been performed on density fractions isolated by conventional ultracentrifugation that are heterogeneous and provide no information about the cargo of apoproteins present in the immunochemically distinct subclasses that populate the density classes. Since apoproteins are now known to have important roles in host defense, we have separated these subclasses according to their apoprotein content and characterized their changes during experimental endotoxemia in human volunteers.
We have studied apoB- and apoA containing lipoprotein subclasses in twelve healthy male volunteers before and for 8 h after a single dose of endotoxin (ET; 2 μg/kg) to stimulate inflammation.
After endotoxin, TG, TC, apoB and the apoB-containing lipoprotein cholesterol-rich subclass LpB and two of the three triglyceride-rich subclasses (TGRLP: Lp:B:C, LpB:C:E+ LpB:E) all declined. In contrast, the third TGRLP, LpA-II:B:C:D:E ("complex particle"), after reaching a nadir at 4 h rose 49% above baseline, p = .006 at 8 h and became the dominant particle in the TGRLP pool. This increment exceeds the threshold of > 25% change required for designation as an acute phase protein. Simultaneous decreases in LpA-I:A-II and LpB:C:E + LpB:E suggest that these subclasses undergo post-translational modification and contribute to the formation of new LpA-II:B:C:D:E particles.
We have identified a new acute phase lipoprotein whose apoprotein constituents have metabolic and immunoregulatory properties applicable to host defense that make it well constituted to engage in the APR.