Modulatory Effect of Beneficial Enterococci and Their Enterocins on the Blood Phagocytes in Murine Experimental Trichinellosis

• Published in: Life (Basel, Switzerland) Vol. 13; no. 9; p. 1930
• Main Authors: Vargová, Miroslava, Revajová, Viera, Lauková, Andrea, Hurníková, Zuzana, Dvorožňáková, Emília
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-09-2023; MDPI
• Subjects: Antimicrobial agents; Antiparasitic agents; Bacteria; Bacteriocins; Biological products; Blood; Care and treatment; durancin-like; enterocin M; Enterocins; Enterococcus; Enzymatic activity; Enzymes; Experiments; Flow cytometry; Gastrointestinal diseases; Granulocytes; Health aspects; Infections; Ingestion; Intestine; Kinases; Laboratory animals; Larvae; Muscles; Parasites; Pathogens; Phagocytes; Phagocytosis; Probiotics; Proteins; Respiratory burst; Small intestine; Strains (organisms); Testing; Trichinella spiralis; Trichinellosis; Trichinosis; Germany; Slovakia; Czech Republic
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life13091930

Bacteriocins (enterocins) represent a new therapeutic strategy in various intestinal and non-intestinal infections. In antiparasitic defence, an oxidative inflammation of phagocytes is effective in destroying new-born Trichinella spiralis larvae. The strains Enterococcus faecium CCM8558 and E. durans ED26E/7 and their enterocins, enterocin M and a durancin-like enterocin, respectively, were administered daily, and mice were then infected with T. spiralis larvae on the seventh day of treatment. Phagotest and Bursttest kits were used to detect the phagocytosis and respiratory burst in blood leukocytes. T. spiralis infection inhibited phagocytosis from day 11 post-infection (dpi) during the migration of new-born larvae into the muscles. E. faecium CCM8558, E. durans ED26E/7, and the durancin-like enterocin increased phagocytic activity from day 11 dpi. Both strains and their enterocins (enterocin M and durancin-like) stimulated the ingestion capability of phagocytes from 18 to 32 dpi. Enterococci/enterocins therapy prevented a reduction in cells with respiratory burst caused by T. spiralis infection from 11 dpi. The enzymatic activity of phagocytes was stimulated on 18 and 25 dpi, particularly by E. faecium CCM8558 and enterocin M. Enterocin M and the durancin-like enterocin were as effective in stimulating phagocytosis as the bacterial strains that produce them. The stimulation of phagocytosis could contribute to decreased larval migration and reduced parasite burden in the host.