Comprehensive Volatilome Signature of Various Brassicaceae Species

• Published in: Plants (Basel) Vol. 12; no. 1; p. 177
• Main Authors: Lukić, Igor, Išić, Nina, Ban, Dean, Salopek Sondi, Branka, Goreta Ban, Smiljana
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-01-2023; MDPI
• Subjects: Alcohol; Analysis; Aroma compounds; Brassica; Brassica oleracea; Brassicaceae; Chemical composition; Chemical properties; Composition; Cruciferae; Cultivars; Diplotaxis tenuifolia; Enzymes; Gas chromatography; glucosinolate hydrolysis; green leaf volatiles; Headspace; Herbivores; isothiocyanates; Kale; Lipoxygenase; lipoxygenase pathway; Liquid oxygen; Mass spectrometry; Mass spectroscopy; Multivariate statistical analysis; Nitriles; Plant resistance; Solid phase methods; Solid phases; Species; Statistical analysis; Varieties; VOCs; Volatile compounds; Volatile organic compounds; Volatiles; volatilome; Croatia; green leaf volatiles; volatilome; glucosinolate hydrolysis; isothiocyanates; Brassica; lipoxygenase pathway
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12010177

To investigate in detail the volatilomes of various Brassicaceae species, landraces, and accessions, and to extract specific volatile markers, volatile aroma compounds were isolated from plant samples by headspace solid-phase microextraction and analyzed by gas chromatography/mass spectrometry (HS-SPME-GC/MS). The data obtained were subjected to uni- and multivariate statistical analysis. In general, two cabbage (
L. var.
) landraces emitted the lowest amounts of volatiles generated in the lipoxygenase (LOX) pathway. Wild species
Ten. and
Vis. were characterized by relatively high
-2-hexenal/
-3-hexen-1-ol ratio in relation to other investigated samples. A Savoy cabbage (
L. var.
) cultivar and three kale (
L. var.
) accessions exhibited particular similarities in the composition of LOX volatiles, while the LOX volatilome fraction of
and
partially coincided with that of another wild species,
L. Regarding volatiles formed in the glucosinolate (GSL) pathway, Savoy cabbage and wild species
,
, and
showed more intense emission of isothiocyanates than cabbage and kale.
showed a rather limited production of nitriles. The results of this study contribute to the general knowledge about volatile composition from various Brassicaceae species, which could be exploited for their better valorization. Future studies should focus on the influence of various environmental, cultivation, and post-harvest factors to obtain data with a higher level of applicability in practice.