Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach

• Published in: Vaccine Vol. 30; no. 49; pp. 7105 - 7110
• Main Authors: Kirk, K, Poh, C.L, Fecondo, J, Pourianfar, H, Shaw, J, Grollo, L
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 19-11-2012
• Subjects: Algorithms; Allergy and Immunology; Animals; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Neutralizing - immunology; Antibodies, Viral - blood; Antibodies, Viral - immunology; Antiviral agents; B-lymphocytes; Capsid protein; Capsid Proteins - genetics; Capsid Proteins - immunology; coat proteins; Computational Biology; Computer applications; Cross Reactions; Economics; Enterovirus; Enterovirus 71; Enterovirus A; Enterovirus A, Human - genetics; Enterovirus A, Human - immunology; Epitopes; Epitopes, B-Lymphocyte - genetics; Epitopes, B-Lymphocyte - immunology; Foot-and-mouth disease; Hand; hand, foot and mouth disease; hidden Markov models; Human enterovirus 71; Humans; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; In silico analysis; Inbreeding; Infection; Learning; Lymphocytes B; Lymphocytes T; Markov chain; Mice; Mice, Inbred BALB C; neutralization tests; neutralizing antibodies; Peptides; Picornaviridae; Poliovirus; Public health; Synthetic peptides; T-lymphocytes; Vaccines; Vaccines, Subunit - administration & dosage; Vaccines, Subunit - chemical synthesis; Vaccines, Subunit - immunology; viruses; Asia; Antibodies; Enterovirus 71; Synthetic peptides; In silico analysis
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.09.030

Highlights ► We used an in silico approach to identify three 15-mer conserved B-cell epitopes. ► We showed that these peptide vaccines induced anti-peptide antibodies. ► We demonstrated that the anti-peptide antibodies could neutralize EV71 in vitro. ► The anti-peptide antibodies were cross-reactive against other Picornaviridae.