New procedure of selected biogenic amines determination in wine samples by HPLC

• Published in: Analytica chimica acta Vol. 834; pp. 58 - 66
• Main Authors: Piasta, Anna M., Jastrzębska, Aneta, Krzemiński, Marek P., Muzioł, Tadeusz M., Szłyk, Edward
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27-06-2014
• Subjects: 2-Chloro-1,3-dinitro-5-(trifluoromethyl)benzene; Amines; Benzene Derivatives - chemistry; Biogenic amines; Biogenic Amines - analysis; Byproducts; Chromatography, High Pressure Liquid - methods; Chromatography, Reverse-Phase - methods; Crystallography, X-Ray; Derivatives; High-performance liquid chromatography; Histamine - analysis; Histamines; Limit of Detection; Liquid chromatography; Magnetic Resonance Spectroscopy; Models, Molecular; Phenethylamines - analysis; Reduction; Spectral and crystallographic analysis; Tryptamines; Tryptamines - analysis; Tyramine - analysis; Wine - analysis; Wine samples; Wines; 2-Chloro-1,3-dinitro-5-(trifluoromethyl)benzene; High-performance liquid chromatography; Wine samples; Biogenic amines; Spectral and crystallographic analysis
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2014.05.028

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•We proposed new procedure for derivatization of biogenic amines.•The NMR and XRD analysis confirmed the purity and uniqueness of derivatives.•Concentration of biogenic amines in wine samples were analyzed by RP-HPLC.•Sample contamination and derivatization reactions interferences were minimized.
A new procedure for determination of biogenic amines (BA): histamine, phenethylamine, tyramine and tryptamine, based on the derivatization reaction with 2-chloro-1,3-dinitro-5-(trifluoromethyl)-benzene (CNBF), is proposed. The amines derivatives with CNBF were isolated and characterized by X-ray crystallography and 1H, 13C, 19F NMR spectroscopy in solution. The novelty of the procedure is based on the pure and well-characterized products of the amines derivatization reaction. The method was applied for the simultaneous analysis of the above mentioned biogenic amines in wine samples by the reversed phase-high performance liquid chromatography. The procedure revealed correlation coefficients (R2) between 0.9997 and 0.9999, and linear range: 0.10–9.00mgL−1 (histamine); 0.10–9.36mgL-1 (tyramine); 0.09–8.64mgL−1 (tryptamine) and 0.10–8.64mgL−1 (phenethylamine), whereas accuracy was 97%–102% (recovery test). Detection limit of biogenic amines in wine samples was 0.02–0.03mgL−1, whereas quantification limit ranged 0.05–0.10mgL−1. The variation coefficients for the analyzed amines ranged between 0.49% and 3.92%. Obtained BA derivatives enhanced separation the analytes on chromatograms due to the inhibition of hydrolysis reaction and the reduction of by-products formation.