Highly Sensitive Fluorescence Detection of Daptomycin in Murine Samples through Derivatization with 2,3-Naphthalenedialdehyde

• Published in: Analytical Sciences Vol. 36; no. 10; pp. 1285 - 1288
• Main Authors: YAMAMOTO, Takuhei, SAKAMOTO, Kousuke, ESAKA, Yukihiro, UNO, Bunji
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 10-10-2020; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: 2,3-naphthalenedialdehyde; Analytical Chemistry; Animals; Calibration; Chemistry; Chromatography, High Pressure Liquid; Daptomycin; Daptomycin - blood; Fluorescence; fluorescent derivatization; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; High performance liquid chromatography; Liquid chromatography; Mice; mice samples; Molecular Conformation; Naphthalenes - chemistry; Ornithine; Ornithine - chemistry; Side effects; high-performance liquid chromatography; 2,3-naphthalenedialdehyde; mice samples; fluorescent derivatization; Daptomycin
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.20N010

A highly sensitive high-performance liquid chromatography method has been developed using the pre-column fluorescent derivatization of daptomycin (DAP) through cyclization of the amino group of ornithine with 2,3-naphthalenedialdehyde. With the proposed method, the limits of detection and quantification of DAP in murine serum were 8 and 3 nmol/L, respectively, and the calibration curve was linear across the examined dynamic range from 8 nmol/L to 1 μmol/L (n = 8, r = 0.9986). This method is suitable for animal experiments examining the side effects of DAP therapy using mice as a simple method with quantification to the order of 10 nmol/L.