continuous isolation of trypsin by affinity adsorbent recycling

A method for the continuous affinity separation of proteins is described in which the adsorbent, in the form of a polymer belt, is recycled through feedstock and eluent liquid flows. As the belt is nonporous, contact between the solute and the ligand is not diffusion-dependent. Consequently, rapid c...

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Bibliographic Details
Published in:	Biotechnology and bioengineering Vol. 56; no. 5; pp. 538 - 545
Main Authors:	Gill, A.L, Niven, G.W, Scurlock, P.G
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 05-12-1997 Wiley
Subjects:	Adsorbents affinity Amino acids Biological and medical sciences Biological Sciences Biotechnology continuous Enzyme engineering Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies protein Proteins Purification Recycling Separation trypsin Purification Serine endopeptidases Bovine Enzyme Continuous process Peptidases Separation method Vertebrata Trypsin Mammalia Hydrolases Affinity Artiodactyla Recycling Adsorbent Pancreas Ungulata
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