Liquid chromatographic procedure for the quantitative analysis of carboplatin in beagle dog plasma ultrafiltrate

• Published in: Journal of pharmaceutical sciences Vol. 77; no. 3; p. 273
• Main Authors: Duncan, G F, Faulkner, 3rd, H C, Farmen, R H, Pittman, K A
• Format: Journal Article
• Language: English
• Published: United States 01-03-1988
• Subjects: Animals; Antineoplastic Agents - blood; Antineoplastic Agents - pharmacokinetics; Carboplatin; Chromatography, Liquid; Dogs; Organoplatinum Compounds - blood; Organoplatinum Compounds - pharmacokinetics; Spectrophotometry, Ultraviolet; Ultrafiltration
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600770318

A specific and reproducible high-performance liquid chromatographic (HPLC) procedure was developed for the quantitative analysis of carboplatin (JM-8) in dog plasma ultrafiltrate. Plasma ultrafiltrate samples were generated using Amicon Centrifree micropartition systems or Amicon Centriflo cones, and injected onto a microBondapak NH2 column. The mobile phase consisted of acetonitrile:methanol:0.005 M sodium perchlorate, pH 2.4 (77-75:13-15:10, v/v/v); the flow rate was 1.5 mL/min. Detection was performed by monitoring UV absorbance of the column effluent at 229 nm. Carboplatin eluted between 9.5 and 11.0 min. The internal standard, JM-10, eluted between 11.0 and 13.0 min. The peak height ratio of carboplatin:internal standard versus carboplatin concentration was linear over a range of 0.2 to 20.0 micrograms/mL. The limit of quantitation was 0.2 microgram/mL. The intra-assay precision of this method, as measured by percent relative standard deviation (%RSD), was within 12% for the theoretical concentrations 0.5, 5.0, and 50.0 micrograms/mL. Accuracies were within 11%. The results of the validation procedures indicated that this procedure was accurate and specific.