Comparison of Antioxidant Capacity and Muscle Amino Acid and Fatty Acid Composition of Nervous and Calm Hu Sheep

• Published in: Antioxidants Vol. 12; no. 2; p. 459
• Main Authors: Zhang, Jinying, Zhang, Yifan, Wang, Jiasheng, Jin, Hengyu, Qian, Shuhan, Chen, Peigen, Wang, Mengzhi, Chen, Ning, Ding, Luoyang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-02-2023; MDPI
• Subjects: amino acid; Amino acid composition; Amino acids; Animal welfare; Antioxidants; Aspartic acid; Catalase; Chromatography; fatty acid; Fatty acid composition; Fatty acids; Glutathione peroxidase; Hu sheep; Kidneys; Lauric acid; Liver; Nutritional aspects; oxidation resistance; Pancreas; Physiological aspects; Sheep; Spleen; Stearic acid; temperament; China; fatty acid; amino acid; oxidation resistance; Hu sheep; temperament
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox12020459

This study determined the effect of temperament on antioxidant capacity and the relationship between antioxidant capacity and the contents of amino acids (AA) and fatty acids (FA) in muscle of Hu sheep. Organ and muscle samples of five calm and five nervous Hu sheep were collected to determine the antioxidant capacity and the contents of AA and FA in muscle tissue. The concentrations of malondialdehyde (MDA) and superoxide excretion enzyme (SOD) in muscle and intestinal tissue of calm Hu sheep were lower than those of nervous Hu sheep (
< 0.01), and the activity of glutathione peroxidase (GSH-Px) in liver of calm Hu sheep was significantly higher than that of nervous Hu sheep (
= 0.050). The content of AA of calm Hu sheep was higher than that of nervous Hu sheep, especially the content of reductive amino acids, which was significantly higher than that of nervous Hu sheep (
= 0.029). Fatty acid content of nervous Hu sheep was higher than that of calm type, and saturated fatty acid content was significantly higher than that of calm type (
= 0.001). The SOD content in muscle tissue was positively correlated with the contents of aspartic acid (Asp), alanine (Ala) and lysine (Lys). Catalase (CAT) activity was positively correlated with Ala content. There was a significant positive correlation between total antioxidants (T-AOC) and glutamate (Glu) (
< 0.05). MDA concentration was positively correlated with lauric acid (C12:0), triseconic acid (C13:0), myristic acid (C14:0) content (
< 0.01), and ginkgo acid (C15:0) content. The total antioxidants (T-AOC) was negatively correlated with stearic acid (C18:0) (
< 0.05). Our conclusion is that the antioxidant capacity of calm Hu sheep is superior to that of nervous Hu sheep, which may be due to the higher AA (especially reductive amino acids (Arg, Lys, Ala and Glu)) content in the muscle and the lower FA (especially SFA) content, which improve the antioxidant capacity of the organism and allow for further exploration of the mechanisms by which animal temperament affects antioxidant performance.