Human Chromosome 16 Encodes a Factor Involved in Induction of Class II Major Histocompatibility Antigens by Interferon γ

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 14; pp. 6077 - 6081
• Main Authors: Bono, M. R., Alcaide-Loridan, C., Couillin, P., Letouze, B., Grisard, M. C., Jouin, H., Fellous, M.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15-07-1991; National Acad Sciences
• Subjects: 550400 - Genetics; Animals; ANTIBODIES; Antibodies, Monoclonal; ANTIGENS; B lymphocytes; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BIOLOGICAL EFFECTS; Blotting, Northern; Cell Line; Cell lines; CHROMOSOMES; Chromosomes, Human, Pair 16; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression Regulation; Genes, MHC Class II - drug effects; GENETIC MAPPING; GROWTH FACTORS; Histocompatibility antigens (hla, h-2 and other systems); Histocompatibility Antigens Class I; Histocompatibility antigens class II; HISTOCOMPATIBILITY COMPLEX; HLA-D Antigens - analysis; HLA-D Antigens - genetics; HUMAN CHROMOSOME 16; HUMAN CHROMOSOME 21; HUMAN CHROMOSOMES; Humans; Hybrid Cells - cytology; Hybrid Cells - immunology; Hybridity; INTERFERON; Interferon-gamma - pharmacology; LYMPHOKINES; MAPPING; Mice; MITOGENS; Molecular immunology; MONOCLONAL ANTIBODIES; ORGANIC COMPOUNDS; PROTEINS; Receptors; Recombinant Proteins; Somatic cells; Transfection; Human; E16-Chromosome; Hybrid cell; Induction; Rodentia; Class II histocompatibility antigen; Cytokine; Northern blotting; Vertebrata; Mammalia; Cell line; Mouse; Immunofluorescence; Gamma interferon
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.14.6077

Interferon γ (IFN-γ) induces expression of class II major histocompatibility complex (MHC)-encoded antigens in immunocompetent cells. To gain further insight into the mechanism of this induction, we prepared somatic cell hybrids between different human cell lines and a murine cell line, RAG, that does not express murine class II MHC antigens before or after treatment with murine IFN-γ. Some of the resulting cell hybrids express murine class II MHC antigens when treated with murine IFN-γ. This inducible phenotype is correlated with the presence of human chromosome 16. It has been shown previously that the induction of class I MHC antigens by human IFN-γ in human-rodent hybrids requires the presence of species-specific factors encoded by chromosome 6, which bears the gene for the human IFN-γ receptor, and chromosome 21, whose product(s) is necessary for the transduction of human IFN-γ signals. In this report, we show that the induction of murine class II MHC antigens by human IFN-γ in the human-RAG cell hybrids requires, likewise, the presence of human chromosomes 6 and 21, in addition to chromosome 16. In some of these hybrids, when all three of these human chromosomes were present, induction of cell-surface HLA-DR antigens was also observed. Our results demonstrate that human chromosome 16 encodes a non-species-specific factor involved in the induction of class II MHC antigens by IFN-γ.