Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells

• Published in: Journal of integrative plant biology Vol. 57; no. 1; pp. 93 - 105
• Main Authors: Sakai, Yuuki, Inoue, Shin‐Ichiro, Harada, Akiko, Shimazaki, Ken‐Ichiro, Takagi, Shingo
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Blackwell Pub 2015; Blackwell Publishing Ltd; Department of Biological Sciences, Graduate School of Science, 0saka University, 0saka 560-0043, Japan%Division of Biological Science, Graduate School of Science, Nagoya University, Aichi 464-8602, Japan%Department of Biology, 0saka Medical Col ege, 0saka 569-8686, Japan%Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-8581, Japan
• Subjects: Amino Acid Sequence; antibodies; Antibodies - metabolism; Avoidance response of chloroplasts; blue light; blue-light response; calcium; Calcium - metabolism; Cell Membrane - metabolism; Cell Membrane - radiation effects; chloroplast de-anchoring; chloroplasts; Chloroplasts - metabolism; Chloroplasts - radiation effects; Cross Reactions; cytoplasmic calcium; genes; Genes, Plant; Hydrocharitaceae - cytology; Hydrocharitaceae - genetics; Hydrocharitaceae - radiation effects; immunoblotting; Intracellular Space - metabolism; irradiation; leaves; Light; Molecular Sequence Data; Molecular Weight; Peptides - metabolism; phosphorylation; Phosphorylation - radiation effects; photoreceptors; phototropin; Phototropins - chemistry; Phototropins - metabolism; Plant Cells - metabolism; Plant Cells - radiation effects; Plant Epidermis - cytology; Plant Epidermis - radiation effects; plasma membrane; polypeptides; Sequence Alignment; Vallisneria; 光诱导; 叶绿体; 叶表皮细胞; 水生被子植物; 磷酸化; 细胞内钙库; 蓝色; 锚固; cytoplasmic calcium; Avoidance response of chloroplasts; chloroplast de-anchoring; blue-light response; phototropin
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/jipb.12284

In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces “chloroplast de‐anchoring”, a rapid decline in the resistance of chloroplasts against centrifugal force. Chloroplast de‐anchoring is known induced within 1 min of irradiation with high‐fluence‐rate blue light specifically, preceding the commencement of chloroplasts migration toward the anticlinal cytoplasm. However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response. In search of the responsible photoreceptors, immunoblotting analysis using antibodies against phototropins demonstrated that cross‐reactive polypeptides of 120‐kDa exist in the plasma‐membrane fraction prepared from the leaves. In vitro phosphorylation analysis revealed that 120‐kDa polypeptides were phosphorylated by exposure to blue light in a fluence‐dependent manner. The blue‐light‐induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness. Furthermore, phototropin gene homologs (Vallisneria PHOTOTROPIN1 and PHOTOTROPIN2) expressed in leaves were isolated. We propose that calcium‐regulated chloroplast de‐anchoring, possibly mediated by phototropins, is an initial process of the blue‐light‐induced avoidance response of chloroplasts in Vallisneria.