Effect of Microplastics on the Activity of Digestive and Oxidative-Stress-Related Enzymes in Peled Whitefish ( Coregonus peled Gmelin) Larvae

• Published in: International journal of molecular sciences Vol. 24; no. 13; p. 10998
• Main Authors: Frank, Yulia A, Interesova, Elena A, Solovyev, Mikhail M, Xu, Jiayi, Vorobiev, Danil S
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-07-2023; MDPI
• Subjects: Aminopeptidase; Analysis; Animals; Antioxidants; Antioxidants - pharmacology; Aquatic environment; Aquatic organisms; Bioindicators; Biomarkers; Catalase; Contamination; Coregonus peled; Ecosystem; Enzymatic activity; Enzyme activity; Enzymes; Esterases; Exposure; Fish; fish larvae; Fishes; Fluorescence; Food contamination; Fresh water; Freshwater ecosystems; Freshwater environments; freshwater fish; Indicators (Biology); Ingestion; Larva - metabolism; Larvae; Metabolism; microplastics; Microplastics - toxicity; Microspheres; Nutritive value; Organisms; Oxidative Stress; peled whitefish; Physiology; Plastic pollution; Plastics; Political aspects; Polystyrene resins; Polystyrenes - pharmacology; PS microspheres; Salmonidae; Water Pollutants, Chemical - analysis; α-Amylase; Germany; Russia; Ob River; fish larvae; enzymatic activity; microplastics; antioxidant enzymes; PS microspheres; freshwater fish; digestive enzymes; peled whitefish
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241310998

Microplastics (MPs) are emergent pollutants in freshwater environments and may impact aquatic organisms, including those of nutritional value. The specific activities of digestive and antioxidant enzymes can be used as good bioindicators of the potential effects of MPs on fish in case of waterborne MP contamination. In this study, we used fluorescent polystyrene microplastics (PS-MPs) to analyze the alterations in enzyme activities in larvae of
Gmelin (peled or Northern whitefish), one of the most valuable commercial fish species of Siberia. Our results indicate that peled larvae can ingest 2 µm PS microspheres in a waterborne exposure model. A positive correlation (
= 0.956;
< 0.01) was found between MP concentration in water and the number of PS microspheres in fish guts, with no significant differences between 24 h and 6-day exposure groups. The ingestion of MPs caused alterations in digestive enzyme activity and antioxidant responses at the whole-body level. The presence of PS-MPs significantly stimulated (
< 0.05) the specific activity of α-Amylase and non-specific esterases in peled larvae after 24 h. However, a pronounced positive effect (
< 0.05) of MPs on the activity of pancreatic trypsine and bile salt-activated lipase was only found after 6 days of exposure compared to after 24 h. Intestinal membrane enzyme aminopeptidase N was also stimulated in the presence of PS-MPs after 6-day exposure. We also observed a significant increase in the specific activity of catalase in peled larvae after 6 days of exposure, which indicates the MP-induced modulation of oxidative stress. Taken together, these results highlight the potential impact of environmental MPs on northern commercial fish, their importance for estimating fish stocks, and the sustainability of freshwater ecosystems.