Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach

Purpose Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its...

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Published in:	Proteomics. Clinical applications Vol. 8; no. 11-12; pp. 916 - 923
Main Authors:	Fajardo Bonin, Renata, Chapeaurouge, Alex, Perales, Jonas, da Silva Jr, José Godinho, do Nascimento, Hilton Jorge, D'Alincourt Carvalho Assef, Ana Paula, Moreno Senna, José Procópio
Format:	Journal Article
Language:	English
Published:	Germany Blackwell Publishing Ltd 01-12-2014 Wiley Subscription Services, Inc
Subjects:	Acinetobacter baumannii Acinetobacter baumannii - immunology Acinetobacter baumannii - physiology Acinetobacter Infections - blood Acinetobacter Infections - immunology Acinetobacter Infections - microbiology Bacteria Bacterial infections Bacterial Outer Membrane Proteins - blood Bacterial Outer Membrane Proteins - immunology Bacterial Proteins - blood Bacterial Proteins - immunology Blotting, Western Electrophoresis, Gel, Two-Dimensional Hospital infection Host-Pathogen Interactions - immunology Humans Immunoproteomics Immunotherapy Infections MALDI-TOF/TOF Proteins Proteome - immunology Proteomics - methods Receptors, Cell Surface - blood Receptors, Cell Surface - immunology Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Hospital infection MALDI-TOF/TOF Acinetobacter baumannii Immunoproteomics
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Abstract	Purpose Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome‐based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii, OMPs were separated by 2DE, and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI‐TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB‐like, OXA‐23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections.
AbstractList	Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii, OMPs were separated by 2DE, and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections. Purpose Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii, OMPs were separated by 2DE, and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections. PURPOSEAcinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection.EXPERIMENTAL DESIGNSera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii, OMPs were separated by 2DE, and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins.RESULTSThis analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth.CONCLUSIONS AND CLINICAL RELEVANCEOur results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections. Purpose Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome‐based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii, OMPs were separated by 2DE, and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI‐TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB‐like, OXA‐23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections.
Author	Fajardo Bonin, Renata do Nascimento, Hilton Jorge Chapeaurouge, Alex Moreno Senna, José Procópio da Silva Jr, José Godinho Perales, Jonas D'Alincourt Carvalho Assef, Ana Paula
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Snippet	Purpose Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised... Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised... Purpose Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised... PURPOSEAcinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised...
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SubjectTerms	Acinetobacter baumannii Acinetobacter baumannii - immunology Acinetobacter baumannii - physiology Acinetobacter Infections - blood Acinetobacter Infections - immunology Acinetobacter Infections - microbiology Bacteria Bacterial infections Bacterial Outer Membrane Proteins - blood Bacterial Outer Membrane Proteins - immunology Bacterial Proteins - blood Bacterial Proteins - immunology Blotting, Western Electrophoresis, Gel, Two-Dimensional Hospital infection Host-Pathogen Interactions - immunology Humans Immunoproteomics Immunotherapy Infections MALDI-TOF/TOF Proteins Proteome - immunology Proteomics - methods Receptors, Cell Surface - blood Receptors, Cell Surface - immunology Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Title	Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
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