Nitric Oxide Donor Modulates a Multispecies Oral Bacterial Community—An In Vitro Study

• Published in: Microorganisms (Basel) Vol. 7; no. 9; p. 353
• Main Authors: Nambu, Takayuki, Wang, Dan, Mashimo, Chiho, Maruyama, Hugo, Kashiwagi, Kosuke, Yoshikawa, Kazushi, Yamamoto, Kazuyo, Okinaga, Toshinori
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 14-09-2019; MDPI
• Subjects: 16S rRNA gene; Antimicrobial agents; Bacteria; Blood pressure; Denitrification; Dental floss; Dental plaque; Environmental factors; Exposure; high-throughput sequencing; Microbiomes; Microbiota; Microorganisms; Nitric oxide; Nitric-oxide synthase; oral microbiota; Pathogens; Physiology; Ribosomal DNA; rRNA 16S; Single-nucleotide polymorphism; Sodium nitroprusside; Tetrazolium salt; Thermal cycling; United States > US; Japan; Germany
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms7090353

The deterioration of human oral microbiota is known to not only cause oral diseases but also to affect systemic health. Various environmental factors are thought to influence the disruption and restoration of the oral ecosystem. In this study, we focused on the effect of nitric oxide (NO) produced by denitrification and NO synthase enzymes on dental plaque microbiota. Interdental plaques collected from 10 subjects were exposed to NO donor sodium nitroprusside (SNP) and then cultured in a specialized growth medium. Depending on the concentration of exposed SNP, a decrease in α-diversity and a continuous change in β-diversity in the dental plaque community were shown by sequencing bacterial 16S rRNA genes. We also identified eight operational taxonomic units that were significantly altered by NO exposure. Among them, the exposure of NO donors to Fusobacterium nucleatum cells showed a decrease in survival rate consistent with the results of microbiota analysis. Meanwhile, in addition to NO tolerance, an increase in the tetrazolium salt-reducing activity of Campylobacter concisus cells was confirmed by exposure to SNP. This study provides an overview of how oral plaque microbiota shifts with exposure to NO and may contribute to the development of a method for adjusting the balance of the oral microbiome.