Effective Biocorrosive Control in Oil Industry Facilities: 16S rRNA Gene Metabarcoding for Monitoring Microbial Communities in Produced Water

• Published in: Microorganisms (Basel) Vol. 11; no. 4; p. 846
• Main Authors: Dutra, Joyce, García, Glen, Gomes, Rosimeire, Cardoso, Mariana, Côrtes, Árley, Silva, Tales, de Jesus, Luís, Rodrigues, Luciano, Freitas, Andria, Waldow, Vinicius, Laguna, Juliana, Campos, Gabriela, Américo, Monique, Akamine, Rubens, de Sousa, Maíra, Groposo, Claudia, Figueiredo, Henrique, Azevedo, Vasco, Góes-Neto, Aristóteles
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-03-2023; MDPI
• Subjects: Anaerobic digestion; Archaea; Bacteria; Bacterial corrosion; Biodegradation; Biological activity; Biological monitoring; Biomarkers; Causes of; Corrosion; Corrosion and anti-corrosives; Deoxyribonucleic acid; DNA; DNA barcoding; Enhanced oil recovery; Environmental aspects; Identification and classification; Laboratories; metabarcoding; Methods; Microbial activity; Microbial colonies; microbiologically influenced corrosion; Microbiomes; Microbiota; Microorganisms; Minimum inhibitory concentration; Monitoring; Monitoring methods; Oil and gas industry; oil industry; Petroleum; Petroleum industry; Petroleum pipelines; Phylogeny; Physiological aspects; Pipe lines; Pipelines; produced water; Production management; rRNA 16S; Sodium; Sulfate reduction; Sulfate-reducing bacteria; Brazil; United States > US; metabarcoding; microbiologically influenced corrosion; produced water; petroleum; oil industry
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11040846

Microbiologically influenced corrosion (MIC) or biocorrosion is a complex biological and physicochemical process, Strategies for monitoring MIC are frequently based on microbial cultivation methods, while microbiological molecular methods (MMM) are not well-established in the oil industry in Brazil. Thus, there is a high demand for the development of effective protocols for monitoring biocorrosion with MMM. The main aim of our study was to analyze the physico-chemi- cal features of microbial communities occurring in produced water (PW) and in enrichment cultures in oil pipelines of the petroleum industry. In order to obtain strictly comparable results, the same samples were used for both culturing and metabarcoding. PW samples displayed higher phylogenetic diversity of bacteria and archaea whereas PW enrichments cultures showed higher dominance of bacterial MIC-associated genera. All samples had a core community composed of 19 distinct genera, with MIC-associated
as the dominant genus. We observed significant associations between the PW and cultured PW samples, with a greater number of associations found between the cultured sulfate-reducing bacteria (SRB) samples and the uncultured PW samples. When evaluating the correlation between the physicochemical characteristics of the environment and the microbiota of the uncultivated samples, we suggest that the occurrence of anaerobic digestion metabolism can be characterized by well-defined phases. Therefore, the detection of microorganisms in uncultured PW by metabarcoding, along with physi-cochemical characterization, can be a more efficient method compared to the culturing method, as it is a less laborious and cost-effective method for monitoring MIC microbial agents in oil industry facilities.