Association of mRNA Expression Levels of LRP1 and Actin-Binding Proteins with the Development of Laryngeal and Laryngopharyngeal Squamous Cell Carcinoma

• Published in: Bulletin of experimental biology and medicine Vol. 169; no. 6; pp. 802 - 805
• Main Authors: Kakurina, G. V., Kolegova, Е. S., Shashova, Е. Е., Velikaya, V. V., Startseva, Zh. А., Cheremisina, О. V., Kondakova, I. V., Choinzonov, Е. L.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-10-2020; Springer; Springer Nature B.V
• Subjects: Actin; Adenylate cyclase; Analysis; Binding proteins; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cofilin; Development and progression; Dysplasia; Endocytosis; Ezrin; Gene expression; Internal Medicine; Laboratory Medicine; Laryngeal cancer; Laryngitis; Medical research; Medicine, Experimental; Messenger RNA; Metastases; Muscle proteins; Oncology; Pathology; Profilin; Protein binding; Reverse transcription; Squamous cell carcinoma; Throat cancer; progression; receptor-mediated endocytosis; LDL receptor-related protein 1; laryngeal and laryngopharyngeal squamous cell carcinoma; actin-binding protein
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-020-04983-7

We analyzed the association of the level of mRNA expression of the main endocytosis receptor LRP1 and actin-binding proteins (ezrin, profilin-1, cofilin-1, and adenylyl cyclase-associated protein 1) with the development and metastasis of laryngeal and laryngopharyngeal squamous cell carcinoma. The mRNA expression was evaluated in paired tissue samples using quantitative reverse transcription real-time PCR (RT-qPCR) and SYBR Green reagents. The study included 38 patients with stage T1-4N0-1M0 laryngeal and laryngopharyngeal squamous cell carcinoma and 10 patients with chronic hyperplastic laryngitis or grade II-III epithelial dysplasia. The expression of
LRP1
in patients with laryngeal and laryngopharyngeal squamous cell carcinoma depended on the stage of the tumor process. Against the background of low expression of
LRP1
mRNA, the relationship between cofilin 1 and profilin 1 expression became stronger (
r
=0.08;
p
=0.05) and a correlation between cofilin 1 and esrin expression (
r
=0.7;
p
=0.05) appeared. Studies on a larger patient cohort are required to make a definite conclusion on the role of
LRP1
in the development of laryngeal and laryngopharyngeal squamous cell carcinoma.