A new harvest of fluorescent proteins
In this issue, Shaner et al. describe a veritable cornucopia of new fluorescent proteins, including mCherry, mBanana, mOrange, mStrawberry, mTangerine and mHoneydew. Fluorescent proteins make possible the relatively straightforward study of favorite proteins within living cells, and cell biologists...
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Published in: | Nature biotechnology Vol. 22; no. 12; pp. 1524 - 1525 |
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01-12-2004
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Abstract | In this issue, Shaner et al. describe a veritable cornucopia of new fluorescent proteins, including mCherry, mBanana, mOrange, mStrawberry, mTangerine and mHoneydew. Fluorescent proteins make possible the relatively straightforward study of favorite proteins within living cells, and cell biologists have become increasingly dependent on them as research tools. Why should this latest crop interest the average cell biologist? The limitations of existing fluorescent proteins have direct consequences for experimental design and interpretation. Shaner et al. address some of these drawbacks and present new variants that should extend our capabilities. |
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AbstractList | In this issue, Shaner et al. describe a veritable cornucopia of new fluorescent proteins, including mCherry, mBanana, mOrange, mStrawberry, mTangerine and mHoneydew. Fluorescent proteins make possible the relatively straightforward study of favorite proteins within living cells, and cell biologists have become increasingly dependent on them as research tools. Why should this latest crop interest the average cell biologist? The limitations of existing fluorescent proteins have direct consequences for experimental design and interpretation. Shaner et al. address some of these drawbacks and present new variants that should extend our capabilities. |
Audience | Academic |
Author | Patterson, George H |
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Cites_doi | 10.1038/nbt1037 10.1074/jbc.M304063200 10.1016/0378-1119(92)90691-H 10.1038/nrm976 10.1126/science.8303295 10.1073/pnas.082243699 10.1093/molbev/msh079 10.1038/13657 10.1042/BJ20040321 10.1038/nbt945 10.1126/science.1082520 |
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SubjectTerms | Animals Anthozoa - genetics Anthozoa - metabolism Discosoma Genetic Enhancement - methods Luminescent Proteins - biosynthesis Luminescent Proteins - chemistry Luminescent Proteins - genetics Mutagenesis, Site-Directed Protein Engineering - methods Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Red Fluorescent Protein Spectrometry, Fluorescence - methods Spectrometry, Fluorescence - trends |
Title | A new harvest of fluorescent proteins |
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