Expression of Inflammation/Pain-Related Genes in the Dorsal Root Ganglion following Disc Puncture in Rats

• Published in: Journal of orthopaedic surgery (Hong Kong) Vol. 24; no. 1; pp. 106 - 112
• Main Authors: Fujioka, Yuki, Stahlberg, Anders, Ochi, Mitsuo, Olmarker, Kjell
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-04-2016; Sage Publications Ltd; SAGE Publishing
• Subjects: Animals; Back pain; cells; Disease Models, Animal; Female; ganglia; Ganglia, Spinal - metabolism; Ganglia, Spinal - physiopathology; Gene Expression; Gene Expression Profiling; Inflammation; Inflammation - genetics; Intervertebral Disc - metabolism; Intervertebral Disc - surgery; Kinases; low back pain; Lumbar Vertebrae; nerve root; nucleus-pulposus; Orthopedics; Ortopedi; Pain; Pain - genetics; Proteins; Punctures; Rats; Rats, Sprague-Dawley; receptor; Rodents; spinal; Studies; Surgery; Tumor necrosis factor-TNF; gene expression profiling; ganglia, spinal; low back pain
• ISSN: 2309-4990; 1022-5536; 2309-4990
• DOI: 10.1177/230949901602400124

Purpose.
To determine the expression of inflammation- and pain-related genes at days 1 and 3 in the dorsal root ganglion (DRG) of rats with or without disc puncture, using real-time quantitative polymerase chain reaction (RT-qPCR) with the TaqMan low-density array (TLDA).
Methods.
53 female Sprague-Dawley rats were used. The left facet joint between L4 and L5 was removed, and the DRG and intervertebral disc between the vertebrae were exposed. The L4–5 intervertebral disc was punctured using a 0.4-mm diameter injection needle (disc puncture group) or left unpunctured (sham group). After one or 3 days, the 53 DRGs were harvested, frozen, and assessed for expression of inflammation-related genes. Total RNA was isolated from the DRGs. Expression of 119 genes related to inflammation and pain in the DRG after disc puncture were analysed using RT-qPCR with the TLDA.
Results.
Of the 95 inflammation-related genes, 78 genes were reliably detected. Two genes were significantly up-regulated: cysteinyl leukotriene receptor 1 (CYSLTR1) at day 3 and interleukin 2 receptor gamma (IL2RG) at day 1, and one gene was significantly down-regulated: phospholipase C beta 3 (PLCB3) at day 1. Of the 24 pain-related genes, 18 genes were reliably detected. Two genes were significantly up-regulated: nitric oxide synthase 1 (NOS1) at days 1 and 3 and 5-HT2A receptor (HTR2A) at day 1.
Conclusion.
Disc puncture may elicit changes in the expression of a variety of genes. Gene expression profiling is a useful tool for detecting new potential pharmaceutical targets for spinal pain syndromes.