Evaluation of the Impact of Some Single-Nucleotide Gene Polymorphisms on the Development of Adenomatous Polyps of the Colon in Patients with Irritable Bowel Syndrome

• Published in: The Turkish Journal of Gastroenterology Vol. 34; no. 8; pp. 822 - 830
• Main Authors: Kyrian, Olena, Dorofeyev, Andrey, Derkach, Igor, Zhigal, Yulia
• Format: Journal Article
• Language: English
• Published: Turkey AVES 01-08-2023; Turkish Society of Gastroenterology
• Subjects: Adenomatous Polyps - complications; Adenomatous Polyps - genetics; Analysis; Care and treatment; Case-Control Studies; Complications and side effects; Development and progression; GASTROINTESTINAL TRACT; Gene Frequency; Genes; Genetic aspects; Genetic Predisposition to Disease; Genotype; Humans; Interleukin-10 - genetics; Interleukins; Intestinal polyps; Irritable bowel syndrome; Irritable Bowel Syndrome - complications; Irritable Bowel Syndrome - genetics; Medical research; Medicine, Experimental; Nucleotides; Original; Polymorphism, Single Nucleotide; Polyps (Pathology); Risk factors; Single nucleotide polymorphisms; Toll-Like Receptors - genetics; Ukraine
• ISSN: 1300-4948; 2148-5607
• DOI: 10.5152/tjg.2023.21985

The number of cases of irritable bowel syndrome is growing worldwide, in which adenomatous polyps can develop as a result of microinflammation of the colonic epithelium. Our study was aimed at the identification of the possible effect of single-nucleotide polymorphisms on the risk of the development of irritable bowel syndrome-related colonic adenomatous polyps.
The study involved 187 irritable bowel syndrome patients. The single-nucleotide polymorphisms were investigated by the polymerase chain reaction method and DNA was extracted with the phenol-chloroform: interleukin-1β gene-31C/T (rs1143627), -511C/T (rs16944); interleukin-6 gene-174G/C (rs1800795); interleukin-10 gene-592C/A (rs1800872), -819T/C (rs1800871), -1082A/G (rs1800896); Toll-like receptor-2 gene Arg753Gln (rs5743708); Toll-like receptor-4 gene Thr399ile (rs4986791), Asp299Gly (rs4986790); and metalloproteinase-9 gene-8202A/G (rs11697325). The study of polymorphic loci was checked for compliance with the Hardy- Weinberg equilibrium using Fisher's exact test along with the analyses of the frequency of alleles and the genotypes.
The association of diseases with G allele Toll-like receptor-2 gene Arg753Gln (rs5743708) was revealed in irritable bowel syndrome patients with adenomatous polyps of the colon (P < .0006) and AG single-nucleotide polymorphisms s of Toll-like receptor-2 gene (χ2 = 12.78, P < .002); A allele had a protective effect. The AG genotype metalloproteinase-9 gene-8202A/G (rs11697325) polymorphism in irritable bowel syndrome patients with adenomatous polyps of the colon had a protective effect (P < .05). AA genotype interleukin-10 gene-1082A/G (rs1800896) polymorphism in the irritable bowel syndrome patient (χ2 = 33.97, 4.0E-8) can be considered as the risk for adenomatous polyps of the colon in irritable bowel syndrome.
G allele Toll-like receptor-2 gene Arg753Gln (rs5743708) and AA genotype interleukin-10 gene-1082A/G (rs1800896) polymorphisms can be the marker of the emergence of adenomatous polyps of the colon concomitant with irritable bowel syndrome.