NMR Structural Characterization of Substrates Bound to N-Acetylglucosaminyltransferase V

N-Acetylglucosaminyltransferase V (GnT-V) is an enzyme involved in the biosynthesis of asparagine-linked oligosaccharides. It is responsible for the transfer of N-acetylglucosamine (GlcNAc) from the nucleotide sugar donor, uridine 5’-diphospho-N-acetylglucosamine (UDP–GlcNAc), to the 6 position of t...

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Bibliographic Details
Published in:	Journal of molecular biology Vol. 366; no. 4; pp. 1266 - 1281
Main Authors:	Macnaughtan, Megan A., Kamar, Maria, Alvarez-Manilla, Gerardo, Venot, Andre, Glushka, John, Pierce, J. Michael, Prestegard, James H.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 02-03-2007
Subjects:	Carbohydrate Conformation Carbohydrate Sequence Epitopes - metabolism glycosyltransferase Magnetic Resonance Spectroscopy - methods Models, Molecular Molecular Sequence Data Molecular Structure N-Acetylglucosaminyltransferases - chemistry NOE Oligosaccharides - chemistry spin-label STD Substrate Specificity UDP-N-acetylglucosamine UDP Km STD NOE UDP > TEMPOH spin-label TEMPOH UDP > TEMPO GnT-V trNOE Gal glycosyltransferase Kd UDP-N-acetylglucosamine NOESY GlcNAc UDP > GlcNAc TEMPO HSQC T1 Glc
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