Global variation in the genetic and biochemical basis of diamondback moth resistance to Bacillus thuringiensis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 94; no. 24; pp. 12780 - 12785
• Main Authors: Tabashnik, B.E, Liu, Y.B, Malvar, T, Heckel, D.G, Masson, L, Ballester, V, Granero, F, Mensua, J.L, Ferre, J
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 25-11-1997; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences of the USA
• Subjects: ALLELES; Animals; BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SUBSP. ENTOMOCIDU; Bacillus thuringiensis Toxins; Bacteria; BACTERIAL PESTICIDES; Bacterial Proteins - metabolism; BACTERIAL TOXINS; BINDING; Bioassay; BIOASSAYS; Biochemistry; Biological Sciences; BRUSH BORDER MEMBRANE VESICLES; Butterflies & moths; Chromosome Mapping; COMPLEMENTATION; CORRELACION GENETICA; CORRELATION GENETIQUE; CROISEMENT; CROSSBREEDING; CRUZAMIENTO; CRYSTAL PROTEINS; Endotoxins - metabolism; ENSAYO BIOLOGICO; Female; FILIPINAS; GENE; GENE RECESSIF; GENES; GENES RECESIVOS; Genetic Complementation Test; GENETIC CORRELATION; GENETIC INHERITANCE; Genetic loci; Genetic mutation; GENETIC RESISTANCE; Genetic Variation; GENETICA; GENETICS; GENETIQUE; Genomic Imprinting; HAWAI; HAWAII; Hemolysin Proteins; HEREDITE; HERENCIA GENETICA; Insect pests; INSECTICIDAL PROPERTIES; INSECTICIDAS; INSECTICIDE; INSECTICIDES; INTESTIN; INTESTINES; INTESTINOS; Larvae; LOCI; LOCUS; MEMBRANA MUCOSA; MEMBRANES; MIDGUT; MORTALIDAD; MORTALITE; MORTALITY; Moths; Moths - genetics; Moths - metabolism; MUCOUS MEMBRANE; MUQUEUSE; MUTACION; MUTATION; PENNSYLVANIA; PENNSYLVANIE; Pest Control, Biological; PESTICIDE BACTERIEN; Pesticides; PHILIPPINES; PLAGUICIDAS BACTERIANOS; PLUTELLA XYLOSTELLA; Protein Binding; PROTEINAS; PROTEINE; PROTEINS; RECESSIVE GENES; RECESSIVE INHERITANCE; RESISTANCE AUX PRODUITS CHIMIQUES; RESISTANCE GENETIQUE; RESISTANCE TO CHEMICALS; RESISTENCIA A PRODUCTOS QUIMICOS; RESISTENCIA GENETICA; STRAINS; TEST BIOLOGIQUE; TOXINAS BACTERIANAS; TOXINE BACTERIENNE; Toxins
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.94.24.12780

Insecticidal proteins from the soil bacterium Bacillus thuringiensis (Bt) are becoming a cornerstone of ecologically sound pest management. However, if pests quickly adapt, the benefits of environmentally benign Bt toxins in sprays and genetically engineered crops will be short-lived. The diamondback moth (Plutella xylostella) is the first insect to evolve resistance to Bt in open-field populations. Here we report that populations from Hawaii and Pennsylvania share a genetic locus at which a recessive mutation associated with reduced toxin binding confers extremely high resistance to four Bt toxins. In contrast, resistance in a population from the Philippines shows multilocus control, a narrower spectrum, and for some Bt toxins, inheritance that is not recessive and not associated with reduced binding. The observed variation in the genetic and biochemical basis of resistance to Bt, which is unlike patterns documented for some synthetic insecticides, profoundly affects the choice of strategies for combating resistance