Hurricane María drives increased indoor proliferation of filamentous fungi in San Juan, Puerto Rico: a two-year culture-based approach

• Published in: PeerJ (San Francisco, CA) Vol. 10; p. e12730
• Main Authors: Vélez-Torres, Lorraine N, Bolaños-Rosero, Benjamín, Godoy-Vitorino, Filipa, Rivera-Mariani, Felix E, Maestre, Juan P, Kinney, Kerry, Cavallin, Humberto
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 03-03-2022; PeerJ Inc
• Subjects: Aeroallergens; Air Microbiology; Analysis; Biodiversity; Cell Proliferation; Climate Change Biology; Cyclonic Storms; Environmental Contamination and Remediation; Environmental Impacts; Environmental Monitoring - methods; Fungi; Humans; Hurricane Maria; Hurricanes; Indoor air; Louisiana; Microbiology; Mold spores; Multiculturalism; Mycobiome; Puerto Rico; Spores, Fungal; Puerto Rico; Louisiana; Flooded homes; Fungi; Aspergillus; Hurricane Maria; Mycobiome; Aeroallergens; Built environment; Indoor environment; Mold spores; Indoor air
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.12730

Extensive flooding caused by Hurricane María in Puerto Rico (PR) created favorable conditions for indoor growth of filamentous fungi. These conditions represent a public health concern as contamination by environmental fungi is associated with a higher prevalence of inflammatory respiratory conditions. This work compares culturable fungal spore communities present in homes that sustained water damage after Hurricane María to those present in dry, non-flooded homes. We collected air samples from 50 houses in a neighborhood in San Juan, PR, 12 and 22 months after Hurricane María. Self-reported data was used to classify the homes as flooded, water-damage or dry non-flooded. Fungi abundances, composition and diversity were analyzed by culturing on two media. Our results showed no significant differences in indoor fungal concentrations (CFU/m
) one year after the Hurricane in both culture media studied (MEA and G25N). During the second sampling period fungal levels were 2.7 times higher in previously flooded homes (Median = 758) when compared to dry homes (Median = 283), (
-value < 0.005). Fungal profiles showed enrichment of
species inside flooded homes compared to outdoor samples during the first sampling period (FDR-adjusted
-value = 0.05). In contrast, 22 months after the storm, indoor fungal composition consisted primarily of non-sporulated fungi, most likely basidiospores, which are characteristic of the outdoor air in PR. Together, this data highlights that homes that suffered water damage not only have higher indoor proliferation of filamentous fungi, but their indoor fungal populations change over time following the Hurricane. Ultimately, after nearly two years, indoor and outdoor fungal communities converged in this sample of naturally ventilated homes.