FRET-based fluorescent probe for drug assay from amino acid@gold-carbon nanoparticles

• Published in: Analytical and bioanalytical chemistry Vol. 413; no. 4; pp. 1117 - 1125
• Main Authors: Hameed, Mehavesh K., Parambath, Javad B. M., Kanan, Sofian M., Mohamed, Ahmed A.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2021; Springer; Springer Nature B.V
• Subjects: Amino acids; Analytical Chemistry; Biochemistry; Biocompatibility; Carbon; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Composition; Energy transfer; Fluorescence; Fluorescence resonance energy transfer; Fluorescent indicators; Food Science; Gastroesophageal reflux; Gold; Laboratory Medicine; Monitoring/Environmental Analysis; Nanoparticles; Peptic ulcers; Quenching; Ranitidine; Research Paper; Tryptophan; Tyrosine; United Arab Emirates; Förster resonance energy transfer; Ranitidine; Gold-carbon nanoparticles; Fluorescence quenching
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-020-03075-9

Biocompatible and luminescent nanostructures synthesized by capping gold-carbon nanoparticles (HOOC-4-C
6
H
4
-AuNPs) with amino acids tyrosine, tryptophan, and cysteine were used for the quantitative estimation of ranitidine (RNH), a peptic ulcer and gastroesophageal reflux drug. We applied a fluorescence quenching mechanism to investigate the viability of the energy transfer based on gold-carbon nanosensors. Förster resonance energy transfer (FRET) calculations showed a donor–acceptor distance of 1.69 nm (Tyr@AuNPs), 2.27 nm (Trp@AuNPs), and 2.32 nm (Cys@AuNPs). The constant time-resolved fluorescence lifetime measurements supported the static quenching nature. This method was successfully utilized in the detection and quantification of RNH, with a limit of detection (LOD) of 0.174, 0.56, and 0.332 μM for Tyr@AuNP, Trp@AuNP, and Cys@AuNP bioconjugates, respectively. This approach was also successful in the quantification of RNH in spiked serum samples.