Differential Expression and Function of Bicellular Tight Junctions in Skin and Oral Wound Healing

• Published in: International journal of molecular sciences Vol. 21; no. 8; p. 2966
• Main Authors: Leonardo, Trevor R, Shi, Junhe, Chen, Dandan, Trivedi, Harsh M, Chen, Lin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23-04-2020; MDPI
• Subjects: Animals; Biomarkers; Cell lines; Cell Membrane Permeability; Claudin-1 - genetics; Claudin-1 - metabolism; Computational Biology; Datasets; Epithelium; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Genes; Histamine; Homeostasis; Keratinocytes; Keratinocytes - metabolism; Lipopolysaccharides; Mice; Mouth Mucosa - metabolism; Oral cavity; oral mucosa; Palate; Phenotypes; Proteins; siRNA; Skin; Skin - metabolism; tight junction; Tight junctions; Tight Junctions - metabolism; Transcriptome; Wound healing; Wound Healing - genetics; skin; wound healing; epithelium; keratinocytes; oral mucosa; tight junction
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21082966

Bicellular tight junctions are multiprotein complexes that are required for maintenance of barrier function and fence function in epithelial tissues. Wound healing in the oral cavity leads to minimal scar formation compared to the skin, and the precise mechanisms for this regenerative response remain to be elucidated. We hypothesized that oral and skin tissues express a different tight junction repertoire both at baseline and during the wound healing response, and that these molecules may be critical to the differential repair between the two tissues. We re-analyzed a mouse skin and palate epithelium microarray dataset to identify the tight junction repertoire of these tissue types. We then re-analyzed a skin and tongue wound healing microarray dataset to see how expression levels of tight junction genes change over time in response to injury. We performed in vitro scratch assays on human oral and skin keratinocyte cell lines to assay for tight junction expression over time, tight junction expression in response to lipopolysaccharide and histamine treatment, and the effects of siRNA knockdown of claudin 1 or occludin on migration and proliferation. Our data showed that oral and skin epithelium expressed different tight junction genes at baseline and during the wound healing response. Knockdown of claudin 1 or occludin led to changes in proliferation and migration in human skin keratinocytes but not oral keratinocytes. Furthermore, we also showed that skin keratinocytes were more permeable than oral keratinocytes upon histamine treatment. In conclusion, this study highlights a specific subset of functional tight junction genes that are differentially expressed between the oral and skin tissues, which may contribute to the mechanisms leading to distinct healing phenotypes in response to injury in the two tissues.