Codon adaptation-based control of protein expression in C. elegans
We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versio...
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| Published in: | Nature methods Vol. 8; no. 3; pp. 250 - 252 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
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01-03-2011
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| Abstract | We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter. |
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| AbstractList | Changing the codon sequence in
Caenorhabditis elegans
genes allows fine-tuning of transgene expression from high to low expression. The same strategy is likely applicable for
Drosophila melanogaster
and
Saccharomyces cerevisiae
.
We present a method to control protein levels under native genetic regulation in
Caenorhabditis elegans
by using synthetic genes with adapted codons. We found that the force acting on the spindle in
C. elegans
embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any
C. elegans
gene can be designed using our web tool,
C. elegans
codon adapter. We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter. We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter. [PUBLICATION ABSTRACT] |
| Audience | Academic |
| Author | Hyman, Antony A Bringmann, Henrik Pozniakowsky, Andrey Ayloo, Swathi Redemann, Stefanie Schloissnig, Siegfried Ernst, Susanne |
| Author_xml | – sequence: 1 givenname: Henrik surname: Bringmann fullname: Bringmann, Henrik organization: Max Planck Institute for Biophysical Chemistry – sequence: 2 givenname: Stefanie surname: Redemann fullname: Redemann, Stefanie organization: Max Planck Institute of Molecular Cell Biology and Genetics – sequence: 3 givenname: Siegfried surname: Schloissnig fullname: Schloissnig, Siegfried organization: European Molecular Biology Laboratory Heidelberg – sequence: 4 givenname: Susanne surname: Ernst fullname: Ernst, Susanne organization: Max Planck Institute of Molecular Cell Biology and Genetics – sequence: 5 givenname: Andrey surname: Pozniakowsky fullname: Pozniakowsky, Andrey organization: Max Planck Institute of Molecular Cell Biology and Genetics – sequence: 6 givenname: Swathi surname: Ayloo fullname: Ayloo, Swathi organization: Max Planck Institute of Molecular Cell Biology and Genetics – sequence: 7 givenname: Antony A surname: Hyman fullname: Hyman, Antony A organization: Max Planck Institute of Molecular Cell Biology and Genetics |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21278743$$D View this record in MEDLINE/PubMed |
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| Snippet | We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found... Changing the codon sequence in Caenorhabditis elegans genes allows fine-tuning of transgene expression from high to low expression. The same strategy is likely... |
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| SubjectTerms | 631/1647/338/552 631/208/200 Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins - genetics Cell Division - genetics Codon Codon - genetics Embryos Gene expression Gene Expression Regulation Genetic aspects Genetic regulation Genetic Techniques Life Sciences Nematodes Physiological aspects Protein Biosynthesis - genetics Protein research Proteins Proteomics Scientific method |
| Title | Codon adaptation-based control of protein expression in C. elegans |
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