Scanning electron microscopy and atomic force microscopy imaging of solid lipid nanoparticles derived from amphiphilic cyclodextrins

• Published in: European journal of pharmaceutics and biopharmaceutics Vol. 55; no. 3; pp. 279 - 282
• Main Authors: Dubes, Alix, Parrot-Lopez, Hélène, Abdelwahed, Wassim, Degobert, Ghania, Fessi, Hatem, Shahgaldian, Patrick, Coleman, Anthony W.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-05-2003; Elsevier Science; Elsevier
• Subjects: Amphiphilic cyclodextrin; Atomic force microscopy; Biochemistry, Molecular Biology; Biological and medical sciences; Cyclodextrins - chemistry; Life Sciences; Lipids - chemistry; Medical sciences; Microscopy, Atomic Force - methods; Microscopy, Electron, Scanning - methods; Nanotechnology - methods; Scanning electron microscopy; Solid lipid nanoparticule; Surface-Active Agents - chemistry; Amphiphilic cyclodextrin; Atomic force microscopy; Scanning electron microscopy; Solid lipid nanoparticule
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/S0939-6411(03)00020-1

Scanning electron microscopy (SEM) and atomic force microscopy (AFM) have been applied to the imagery of solid lipid nanoparticles (SLNs) formulated from an amphiphilic cyclodextrin, 2,3-di-
o-alkanoyl-β-cyclodextrin, β-CD21C6. Comparison of the results shows that the vacuum drying technique used in sample preparation for SEM causes shrinkage in the size of the SLNs, whereas the deposition method used for AFM causes the SLNs to form small clusters. The hydrodynamic diameter determined from photon correlation spectroscopy (PCS) is 359±15
nm and the zeta potential is −25
mV.