Olfaxin as a novel Prune2 isoform predominantly expressed in olfactory system

• Published in: Brain research Vol. 1488; pp. 1 - 13
• Main Authors: Li, Shimo, Hayakawa-Yano, Yoshika, Itoh, Masanori, Ueda, Masashi, Ohta, Kazunori, Suzuki, Yoshihiro, Mizuno, Akihito, Ohta, Eri, Hida, Yoko, Wang, Miao-xing, Nakagawa, Toshiyuki
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07-12-2012; Elsevier
• Subjects: Alzheimer disease; Amino Acid Sequence; Animals; antagonists; apoptosis; Base Sequence; Biological and medical sciences; biomarkers; Caspase; caspase-3; cerebellum; cortex; cytosol; Cytosol - metabolism; Drosophila; Estriol - analogs & derivatives; Estriol - metabolism; Exons - genetics; Fundamental and applied biological sciences. Psychology; genes; glutamic acid; HEK293 Cells; hippocampus; Humans; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Neoplasm Proteins - chemistry; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurology; neurons; Olfactory bulb; Olfactory Bulb - physiology; Olfactory Pathways - physiology; Olfactory system and olfaction. Gustatory system and gustation; Piriform cortex; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protein Structure, Tertiary - physiology; proteins; Synapse; Vertebrates: nervous system and sense organs; BMCC1; BCH; PRUNE2; Caspase; BNIPXL; BNIP2 extra long; Prune homolog 2; BNIP; BCH-motif-containing molecule at the C-terminal region 1; Synapse; BNIP2, and Cdc42GAP homology; Piriform cortex; Bcl-2/adenovirus E1B 19 kDa-interacting protein; Olfactory bulb; Molecular form; Enzyme; Cysteine endopeptidases; Central nervous system; Olfactory system; Encephalon; Peptidases; Olfactory pathway; Hydrolases
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2012.10.001

Abstract Prune homolog 2 ( Drosophila ) ( PRUNE2 ) encodes a BCH motif-containing protein that shares homology with the Cayman ataxia-related protein Caytaxin. Caytaxin is a substrate of caspase-3 and is specifically expressed at the presynapse of vesicular-type glutamate transporter (VGLUT)-positive neurons, where it plays a role in glutamate neurotransmission primarily in the cerebellum and hippocampus. Here, we showed that a novel Prune2 isoform contains a BCH motif and localizes predominantly to the synaptic cytosol, similar to Caytaxin. However, the isoform is expressed predominantly in the olfactory bulb and layer Ia of the piriform cortex, where Caytaxin is scarcely expressed. The isoform expression is upregulated during development, similar to that in the presynaptic-localizing proteins Synapsin I and Bassoon. Prune2 and its previously identified isoforms have been shown to be a susceptibility gene for Alzheimer's disease, a biomarker for leiomyosarcomas, a proapoptotic protein, and an antagonist of cellular transformation. In addition, a novel isoform may develop new roles for Prune2 at the synapse in olfactory systems.