Screening for Oral Cancer Using Electrochemical Telomerase Assay

Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expres...

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Published in:Electroanalysis (New York, N.Y.) Vol. 28; no. 3; pp. 503 - 507
Main Authors: Hayakawa, Mana, Sato, Shinobu, Diala, Irmina, Kodama, Masaaki, Tomoeda-Mori, Kumiko, Haraguchi, Kazuya, Tominaga, Kazuhiro, Takenaka, Shigeori
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Language:English
Published: Weinheim WILEY-VCH Verlag 01-03-2016
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Abstract Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real‐time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88 % and 72 %, respectively when used 17 % of the threshold value based on the receiver operating characteristic curve in ECTA data.
AbstractList Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real-time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88% and 72%, respectively when used 17% of the threshold value based on the receiver operating characteristic curve in ECTA data.
Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real‐time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88 % and 72 %, respectively when used 17 % of the threshold value based on the receiver operating characteristic curve in ECTA data.
Author Kodama, Masaaki
Tominaga, Kazuhiro
Tomoeda-Mori, Kumiko
Takenaka, Shigeori
Hayakawa, Mana
Haraguchi, Kazuya
Sato, Shinobu
Diala, Irmina
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  givenname: Shinobu
  surname: Sato
  fullname: Sato, Shinobu
  organization: Department of Applied Chemistry, Kyushu Institute of Technology, Fukuoka, Japan
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  givenname: Irmina
  surname: Diala
  fullname: Diala, Irmina
  organization: Department of Applied Chemistry, Kyushu Institute of Technology, Fukuoka, Japan
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  givenname: Masaaki
  surname: Kodama
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  organization: Division of Oral and Maxillofacial Surgery, Department of Science of Physical Functions, Kyushu Dental University, Fukuoka, Japan
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  givenname: Kumiko
  surname: Tomoeda-Mori
  fullname: Tomoeda-Mori, Kumiko
  organization: Department of Oral and Maxillofacial Surgery, National Hospital Organization Kumamoto Medical Center, Kumamoto, Japan
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  organization: Division of Oral and Maxillofacial Surgery, Department of Science of Physical Functions, Kyushu Dental University, Fukuoka, Japan
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  givenname: Kazuhiro
  surname: Tominaga
  fullname: Tominaga, Kazuhiro
  organization: Division of Oral and Maxillofacial Surgery, Department of Science of Physical Functions, Kyushu Dental University, Fukuoka, Japan
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  givenname: Shigeori
  surname: Takenaka
  fullname: Takenaka, Shigeori
  email: shige@che.kyutech.ac.jp
  organization: Department of Applied Chemistry, Kyushu Institute of Technology, Fukuoka, Japan
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Snippet Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from...
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SubjectTerms Assaying
Cancer
Correlation
electrochemical assay
Gene expression
hTERT gene
oral cancer screening
Real time
Receivers
Screening
Telomerase
Title Screening for Oral Cancer Using Electrochemical Telomerase Assay
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