Screening for Oral Cancer Using Electrochemical Telomerase Assay
Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expres...
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Published in: | Electroanalysis (New York, N.Y.) Vol. 28; no. 3; pp. 503 - 507 |
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01-03-2016
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Abstract | Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real‐time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88 % and 72 %, respectively when used 17 % of the threshold value based on the receiver operating characteristic curve in ECTA data. |
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AbstractList | Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real-time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88% and 72%, respectively when used 17% of the threshold value based on the receiver operating characteristic curve in ECTA data. Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real‐time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88 % and 72 %, respectively when used 17 % of the threshold value based on the receiver operating characteristic curve in ECTA data. |
Author | Kodama, Masaaki Tominaga, Kazuhiro Tomoeda-Mori, Kumiko Takenaka, Shigeori Hayakawa, Mana Haraguchi, Kazuya Sato, Shinobu Diala, Irmina |
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SubjectTerms | Assaying Cancer Correlation electrochemical assay Gene expression hTERT gene oral cancer screening Real time Receivers Screening Telomerase |
Title | Screening for Oral Cancer Using Electrochemical Telomerase Assay |
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