Structural Requirements of the Fructan-Lipid Interaction

• Published in: Biophysical journal Vol. 84; no. 5; pp. 3147 - 3154
• Main Authors: Vereyken, Ingrid J., Albert van Kuik, J., Evers, Toon H., Rijken, Pieter J., de Kruijff, Ben
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-05-2003; Biophysical Society
• Subjects: Biochemistry; Cellular biology; Computer Simulation; Crystallography - methods; Fructans - chemistry; Fructans - classification; Glycolipids - chemistry; Lipid Bilayers - chemistry; Lipids; Liposomes - chemistry; Macromolecular Substances; Membrane Fluidity; Membranes; Models, Molecular; Molecular Conformation; Phospholipids - chemistry; Pyrimidinones; Reproducibility of Results; Sensitivity and Specificity; DOPE; MC 540; PMF; LUVETs; PC; PE; MD; MGDG; DGDG; DMPC; DOPENMe2; DOPC; DPPC; AUS
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(03)70039-3

Fructans are a group of fructose-based oligo- and polysaccharides. They are proposed to be involved in membrane protection of plants during dehydration. In accordance with this hypothesis, they show an interaction with hydrated lipid model systems. However, the structural requirements for this interaction are not known both with respect to the fructans as to the lipids. To get insight into this matter, the interaction of several inulins and levan with lipids was investigated using a monomolecular lipid system or the MC 540 probe in a bilayer system. MD was used to get conformational information concerning the polysaccharides. It was found that levan-type fructan interacted comparably with model membranes composed of glyco- or phospholipids but showed a preference for lipids with a small headgroup. Furthermore, it was found that there was an inulin chain-length-dependent interaction with lipids. The results also suggested that inulin-type fructan had a more profound interaction with the membrane than levan-type fructan. MD simulations indicated that the favorable conformation for levan is a helix, whereas inulin tends to form random coil structures. This suggests that flexibility is an important determinant for the fructan-lipid interaction.