Functional analysis of pneumolysin by use of monoclonal antibodies

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Published in:	Infection and Immunity Vol. 64; no. 2; pp. 480 - 484
Main Authors:	DE LOS TOYOS, J. R, MENDEZ, F. J, APARICIO, J. F, VAZQUEZ, F, GARCIA SUAREZ, M. DEL M, FLEITES, A, HARDISSON, C, MORGAN, P. J, ANDREW, P. W, MITCHELL, T. J
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-02-1996
Subjects:	Animals Antibodies, Monoclonal - immunology Bacterial Proteins Bacteriology Biological and medical sciences Erythrocytes - metabolism Female Flow Cytometry Fundamental and applied biological sciences. Psychology Male Mice Mice, Inbred BALB C Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Streptococcus pneumoniae Streptolysins - immunology Streptolysins - physiology Streptococcaceae Toxin Bacteria Micrococcales Monoclonal antibody Mechanism of action Hemolysin Structure function relationship Streptococcus pneumoniae
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Abstract	Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue IAI About IAI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy Connect to IAI IAI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0019-9567 Online ISSN: 1098-5522 Copyright © 2014 by the American Society for Microbiology. For an alternate route to IAI .asm.org, visit: IAI
AbstractList	We have produced a panel of monoclonal antibodies to pneumolysin, the membrane-damaging toxin from Streptococcus pneumoniae. We have used these antibodies to identify three regions of the toxin sequence that are involved in the lytic mechanism of this toxin. Two of these sites probably form the cell binding site of this toxin. Antibodies to the third site inhibit the lytic action of this toxin but not the binding of this toxin to cells. This site is engaged in the oligomerization process involved in the formation of pores in cell membranes. Two of these epitopes are also present in the related toxin perfringolysin O. Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue IAI About IAI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy Connect to IAI IAI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0019-9567 Online ISSN: 1098-5522 Copyright © 2014 by the American Society for Microbiology. For an alternate route to IAI .asm.org, visit: IAI
Author	P W Andrew C Hardisson T J Mitchell M Del Mar García Suárez J F Aparicio F J Méndez J R de los Toyos F Vázquez P J Morgan A Fleites
AuthorAffiliation	Facultad de Medicina, Universidad de Oviedo, Spain
AuthorAffiliation_xml	– name: Facultad de Medicina, Universidad de Oviedo, Spain
Author_xml	– sequence: 1 givenname: J. R surname: DE LOS TOYOS fullname: DE LOS TOYOS, J. R organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 2 givenname: F. J surname: MENDEZ fullname: MENDEZ, F. J organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 3 givenname: J. F surname: APARICIO fullname: APARICIO, J. F organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 4 givenname: F surname: VAZQUEZ fullname: VAZQUEZ, F organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 5 givenname: M. DEL M surname: GARCIA SUAREZ fullname: GARCIA SUAREZ, M. DEL M organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 6 givenname: A surname: FLEITES fullname: FLEITES, A organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 7 givenname: C surname: HARDISSON fullname: HARDISSON, C organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 8 givenname: P. J surname: MORGAN fullname: MORGAN, P. J organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 9 givenname: P. W surname: ANDREW fullname: ANDREW, P. W organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain – sequence: 10 givenname: T. J surname: MITCHELL fullname: MITCHELL, T. J organization: Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain
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SubjectTerms	Animals Antibodies, Monoclonal - immunology Bacterial Proteins Bacteriology Biological and medical sciences Erythrocytes - metabolism Female Flow Cytometry Fundamental and applied biological sciences. Psychology Male Mice Mice, Inbred BALB C Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Streptococcus pneumoniae Streptolysins - immunology Streptolysins - physiology
Title	Functional analysis of pneumolysin by use of monoclonal antibodies
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