Single-Step Recovery of a Secreted Recombinant Protein by Expanded Bed Adsorption

• Published in: Bio/technology (New York, N.Y. 1983) Vol. 12; no. 3; pp. 285 - 288
• Main Authors: Hansson, Marianne, Ståhl, Stefan, Hjorth, Rolf, Uhlén, Mathias, Moks, Tomas
• Format: Journal Article
• Language: English
• Published: New York, NY Nature Publications 01-03-1994
• Subjects: Adsorption; Animals; Anions; Antigens, Protozoan - genetics; Antigens, Protozoan - isolation & purification; Antigens, Surface - genetics; Antigens, Surface - isolation & purification; Base Sequence; Biological and medical sciences; Biotechnology; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli - chemistry; Escherichia coli - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Immunoglobulin G; Isoelectric Point; Methods. Procedures. Technologies; Molecular Sequence Data; Others; Plasmodium falciparum; Protozoan Proteins - genetics; Protozoan Proteins - isolation & purification; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - isolation & purification; Recombinant Fusion Proteins - secretion; Staphylococcal Protein A - genetics; Staphylococcal Protein A - isolation & purification; Various methods and equipments; Culture medium; Purification; Gel electrophoresis; Escherichia coli; IgG; Fermentation; Characterization; Plasmid; Adsorption; Affinity chromatography; Production; Microorganism culture; Anion exchanger; Bacteria; Recombinant protein; Enterobacteriaceae
• ISSN: 0733-222X; 2331-3684
• DOI: 10.1038/nbt0394-285

We have used an expanded bed adsorption procedure for efficient recovery of a recombinant fusion protein, directly from a crude fermentor broth without prior cell removal. The fusion protein was designed to have a relatively low isoelectric point (pI) to allow anionic exchange adsorption at pH 5.5 where most Escherichia coli host proteins are not adsorbed. The gene product was secreted to the culture medium of the E. coli host cells in high yields (550 mg/l). The separation of cells and the concentration and recovery of the fusion protein could therefore be achieved by a single unit operation. The yield after the expanded bed adsorption exceeded 90 percent. Furthermore, the significant volume reduction by the expanded bed adsorption, enabled efficient and straight-forward polishing of the product by a subsequent affinity chromatography step, for removal of contaminating DNA and pyrogenic compounds to levels acceptable for regulatory authorities. An overall yield exceeding 90 percent was maintained after the affinity chromatography polishing step. The procedure outlined here is suitable for large-scale bioprocesses and allows efficient removal of cells, host proteins, contaminating DNA and endotoxins.