WLIP, a lipodepsipeptide of Pseudomonas ‘reactans’, as inhibitor of the symptoms of the brown blotch disease of Agaricus bisporus

• Published in: Journal of applied microbiology Vol. 86; no. 4; pp. 635 - 641
• Main Authors: Soler‐Rivas, C., Arpin, N., Olivier, J. M., Wichers, H. J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-04-1999; Blackwell Science; Wiley
• Subjects: Agaricus bisporus; Agrotechnological Research Institute; Bacterial plant pathogens; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control; Instituut voor Agrotechnologisch Onderzoek; Life Sciences; Microbiology and Parasitology; Phytopathology. Animal pests. Plant and forest protection; Pseudomonas reactans; Pseudomonas tolaasii; Fungi; Pseudomonadales; Agaricus bisporus; Browning; Plant pathogen; Biological control; Bacteria; Basidiomycetes; Pseudomonadaceae; Inhibition; Pseudomonas tolaasii; Thallophyta; EFFET INHIBITEUR
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1046/j.1365-2672.1999.00709.x

A cell‐free crude extract containing the white line inducing principle (WLIP), a lipodepsipeptide produced by Pseudomonas ‘reactans’, could inhibit browning of mushrooms caused by Pseudomonas tolaasii. Mushrooms inoculated with Ps. tolaasii at concentrations of 2·7 × 106 cfu ml−1 or higher showed the symptoms of the disease after 2 d of incubation. Mushroom caps treated with various concentrations of a crude WLIP preparation, and later inoculated with bacterial concentrations higher than the threshold value, did not develop the symptoms of the disease. One milligram of a crude WLIP preparation could block 50% of the symptoms caused by 1·2 × 107 cfu. The inhibition of browning was effective when incubating at low temperatures for 4 d. A suspension containing 1·6 mg ml−1 of pure WLIP was also able to inhibit the symptoms of brown blotch disease induced by 7·6 × 106 cfu ml−1 of Ps. tolaasii.