The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2

The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2

This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA...

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Published in:Scientific reports Vol. 14; no. 1; p. 748
Main Authors: Al-Qaoud, Khaled M., Obeidat, Yusra M., Al-Omari, Tareq, Okour, Mohammad, Al-Omari, Mariam M., Ahmad, Mohammad I., Alshadfan, Raed, Rawashdeh, AbdelMonem M.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 07-01-2024
Nature Publishing Group
Nature Portfolio
Subjects:
631/1647
631/61
692/699
Animals
Antibodies
Antigens
Biosensing Techniques
Biosensors
Blood Group Antigens
Chickens
COVID-19
COVID-19 - diagnosis
Eggs
Electric properties
Electrochemistry
Electrodes
Enzyme-linked immunosorbent assay
Humanities and Social Sciences
Immunization
Immunoassay
Immunosensors
Medical research
multidisciplinary
Proteins
RNA, Viral
SARS-CoV-2
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
Spike protein
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Abstract This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
AbstractList This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
Abstract This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
ArticleNumber 748
Author Al-Omari, Tareq
Al-Qaoud, Khaled M.
Rawashdeh, AbdelMonem M.
Obeidat, Yusra M.
Al-Omari, Mariam M.
Alshadfan, Raed
Okour, Mohammad
Ahmad, Mohammad I.
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  givenname: Raed
  surname: Alshadfan
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  givenname: AbdelMonem M.
  surname: Rawashdeh
  fullname: Rawashdeh, AbdelMonem M.
  organization: Department of Chemistry, Faculty of Science, Yarmouk University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/38185704$$D View this record in MEDLINE/PubMed
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CitedBy_id crossref_primary_10_1002_adom_202400849
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crossref_primary_10_1021_acsami_4c04567
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Snippet This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor...
Abstract This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric...
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SubjectTerms 631/1647
631/61
692/699
Animals
Antibodies
Antigens
Biosensing Techniques
Biosensors
Blood Group Antigens
Chickens
COVID-19
COVID-19 - diagnosis
Eggs
Electric properties
Electrochemistry
Electrodes
Enzyme-linked immunosorbent assay
Humanities and Social Sciences
Immunization
Immunoassay
Immunosensors
Medical research
multidisciplinary
Proteins
RNA, Viral
SARS-CoV-2
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
Spike protein
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Title The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2
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