Effects of high-intensity interval training in more or less active mice on biomechanical, biophysical and biochemical bone parameters

• Published in: Scientific reports Vol. 11; no. 1; p. 6414
• Main Authors: Polisel, Emanuel E. C., Beck, Wladimir R., Scariot, Pedro P. M., Pejon, Taciane M. M., Gobatto, Claudio A., Manchado-Gobatto, Fúlvia B.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19-03-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/443; 631/45/56; 631/535; 631/57; 639/638/45; 692/499; 692/700; Animals; Biomechanics; Bone Density - physiology; Bones; Calcium; Calcium - analysis; Femur; Femur - chemistry; Femur - physiology; High-Intensity Interval Training - adverse effects; Housing, Animal; Humanities and Social Sciences; Interval training; Long bone; Male; Mice; Mice, Inbred C57BL; multidisciplinary; Phosphorus - analysis; Physical activity; Physical Conditioning, Animal - physiology; Physical fitness; Physical Fitness - physiology; Physical training; Science; Science (multidisciplinary); Sedentary Behavior; Variance analysis; Weight-Bearing - physiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-85585-9

High-intensity interval training (HIIT) is of scientific interest due its role in improving physical fitness, but the effects of HIIT on bone health need be carefully explored. Further, it is necessary to know whether HIIT effects on bone health are dependent on the physical activity levels. This may be experimentally tested since we have built a large cage (LC) that allows animals to move freely, promoting an increase of spontaneous physical activity (SPA) in comparison to a small cage (SC). Thus, we examined the effects of HIIT on biophysical, biomechanical and biochemical parameters of bone tissue of C57BL/6J mice living in cages of two different sizes: small (SC) or large (LC) cages with 1320 cm
2
and 4800 cm
2
floor space, respectively. Male mice were subdivided into two groups within each housing type: Control (C) and Trained (T). At the end of the interventions, all mice were euthanized to extract the femur bone for biophysical, biomechanical and biochemical analyses. Based a significant interaction from two-way ANOVA, trained mice kept in large cage (but not for trained mice housed in SC) exhibited a reduction of tenacity and displacement at failure in bone. This suggests that long-term HIIT program, in addition with a more active lifestyle correlates with exerts negative effects on the bone of healthy mice. A caution must also be raised about the excessive adoption of physical training, at least regarding bone tissue. On the other hand, increased calcium was found in femur of mice housed in LC. In line with this, LC-C mice were more active (i.e. SPA) than other groups. This implies that an active lifestyle without long-term high intensity physical training seems to play a role in promoting benefits to bone tissue. Our data provides new insights for treatment of osteo-health related disorders.